PMID: 109118May 9, 1979

Characterisation of reaction centers and their phospholipids from Rhodospirillum rubrum

Biochimica Et Biophysica Acta
M Snozzi, R Bachofen

Abstract

1. Reaction centers from Rhodospirillum rubrum have been extracted with the zwitterionic detergent lauryl dimethyl amine oxide. Subsequent purification has been achieved by gel filtration and ion-exchange chromatography. The pure reaction centers are composed of three protein subunits (L, M, H), bacteriocholorophyll and bacteriopheophytin in the ratio 2 : 1 and phospholipids. 2. The phospholipid composition has been found to be similar to that of whole chromatophore membrane, except that diphosphatidyl glycerol is present in higher amount in the isolated complex. When the detergent treatment of the chromatophore membrane is done in the presence of NaCl, a lower phospholipid content in isolated reaction centers has been found together with a lower stability in the association among the protein subunits. In this complex, the largest subunit H is easily split off and a LM complex is obtained. It is concluded that the phospholipids play an important role in the stability of reaction center complexes.

References

Feb 8, 1977·Biochemistry·N C Robinson, R A Capaldi
Mar 13, 1978·Biochimica Et Biophysica Acta·B J Clayton, R K Clayton
Apr 18, 1977·Biochimica Et Biophysica Acta·D Wildenauer, H G Khorana
Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M Y OkamuraG Feher
Nov 17, 1972·Biochimica Et Biophysica Acta·T Beugeling Barelds-Van de Beek

Citations

Aug 1, 1987·Journal of Bioenergetics and Biomembranes·H R BosshardR Bachofen
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·W LubitzK Möbius
Apr 16, 1985·Biochemical and Biophysical Research Communications·R RiederH R Bosshard
Oct 14, 1987·Biochemical and Biophysical Research Communications·J HladikR Bachofen
Jul 1, 1984·Hoppe-Seyler's Zeitschrift für physiologische Chemie·R TheilerH Zuber

Related Concepts

Bacterial Proteins
Cell Fractionation
Synthetic Detergent
Dimethylamines
Intracellular Membranes
Phospholipids
Pigments, Biological
Rhodospirillum rubrum

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