PMID: 8585988Nov 1, 1995Paper

Characterisation of repeated sequences from microdissected B chromosomes of Crepis capillaris

Chromosoma
M JamilenaJ S Parker

Abstract

The B chromosome of Crepis capillaris was isolated from the standard chromosomes by microdissection, and the chromosomal DNA amplified using the degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR). The PCR product was cloned and a B-specific library created and characterised. Southern and in situ hybridisation analyses of the DOP-PCR product from microdissected B chromosomes confirmed that the B chromosome is composed mainly of sequences also present in the A chromosomes but lacks the main repeated DNA families located in the A-chromosomal heterochromatin. From 100 clones analysed, 12% of the generated B-chromosomal library was shown to be composed of dispersed repeats located in both the A and B chromosomes. No B-specific repeated sequence was detected. One of the most abundant repeated DNAs within the library, the family B134, was further characterised. Repeating units show a sequence similarity range from 69% to 90% and are characterised by their richness in (CA)n repeats. In situ hybridisation revealed that members of this family are dispersed throughout the A and B chromosomes but are more concentrated in the pericentromeric heterochromatin of the B, indicating that the molecular organization of B heteroc...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A WeinrebB K Birshtein
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H HamadaT Kakunaga
Mar 1, 1994·Molecular & General Genetics : MGG·K ArumuganathanE D Earle
Oct 1, 1993·Genetics·M R Alfenito, J A Birchler
Mar 1, 1993·Chromosoma·M JamilenaM Ruiz Rejón
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·W J PeacockA J Pryor
Aug 1, 1993·Genome Génome / Conseil National De Recherches Canada·R BlundenR N Jones

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Citations

Jun 19, 1998·Mutation Research·W F Grant, E T Owens
Mar 21, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J P CamachoL W Beukeboom
Dec 24, 2008·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·María TeruelJuan Pedro M Camacho
Mar 9, 2005·Cytogenetic and Genome Research·A FominayaE Ferrer
Dec 18, 2009·Chromosoma·María TeruelJuan Pedro M Camacho
May 15, 2007·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Qiuhong DengYong Yang
Sep 27, 2014·Molecular Genetics and Genomics : MGG·Kuo-Wei KaoYa-Ming Cheng
Nov 14, 1997·Genome Génome / Conseil National De Recherches Canada·C P ScuttP M Gilmartin
Feb 25, 2006·Genome Génome / Conseil National De Recherches Canada·Beatrice MariottiManuel Jamilena
Oct 27, 2018·Genes·André MarquesAndreas Houben
May 19, 2009·Genome Génome / Conseil National De Recherches Canada·Joke J F A van VugtHans de Jong
Apr 4, 2021·Plants·Martina BednářováJan Bartoš

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