PMID: 9657332Jul 10, 1998Paper

Characterisation of Tc-cpl-1, a cathepsin L-like cysteine protease from Toxocara canis infective larvae

Molecular and Biochemical Parasitology
A LoukasR M Maizels

Abstract

Cysteine proteases play vital biological roles in both intracellular and extracellular environments. A cysteine protease migrating at 30 kDa was identified in somatic extracts of Toxocara canis larvae (TEX), by its binding to the biotinylated inhibitor Phe-Ala-CH2F. TEX proteases readily cleaved the cathepsin L- and B-specific peptide substrate Z-Phe-Arg-AMC and to a lesser extent, the cathepsin B-specific peptide Z-Arg-Arg-AMC. Excretory/secretory (TES) products of T. canis larvae did not cleave either substrate. Partial sequence encoding the 5' end of a cysteine protease cDNA from infective T. canis larvae was then obtained from an expressed sequence tag (EST) project. The entire cDNA (termed Tc-cpl-1) was subsequently sequenced and found to encode a preproenzyme similar to cathepsin L-like proteases (identities between 36 and 69%), the closest homologues being two predicted proteins from Caenorhabditis elegans cosmids, a cathepsin L-like enzyme from Brugia pahangi and a range of parasite and plant papain-like proteases. Sequence alignment with homologues of known secondary structure indicated several charged residues in the S1 and S2 subsites involved in determining substrate specificity. Some of these are shared with human ...Continue Reading

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Citations

Feb 3, 2009·Parasitology Research·Daya Ram PokharelSushma Rathaur
Mar 25, 2000·International Journal for Parasitology·R M MaizelsA Loukas
Jun 22, 2002·Molecular and Biochemical Parasitology·Collette Britton, Linda Murray
Sep 16, 2000·Insect Biochemistry and Molecular Biology·G RenardA Masuda
Sep 6, 2003·Trends in Parasitology·Angela L WilliamsonAlex Loukas
Aug 9, 2016·PLoS Neglected Tropical Diseases·Louise S GoupilJames H McKerrow
May 7, 2009·Molecular and Biochemical Parasitology·Mayuri TarasukRudi Grams
Apr 25, 2007·DNA Sequence : the Journal of DNA Sequencing and Mapping·Charles I MulekeLi Xiangrui

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