PMID: 9546816Jan 1, 1997Paper

Characterisation of the antibody response to the extracellular region of recombinant thyrotropin receptor

Autoimmunity
N G MorgenthalerJ P Banga

Abstract

Autoantibodies to the human thyrotropin receptor (TSH-R) are pathogenic in a number of autoimmune thyroid diseases including Graves' disease. We have characterised polyclonal antisera to TSH-R for antibodies which may mimic those present in autoimmune thyroid disease. For immunisations, recombinant extracellular region of human TSH-R which does not interact with its ligand TSH was used. The induced antibodies react with the full length membrane receptor in transfected mammalian cells by flow cytometry showing the presence of antibody capable of recognising the native functional receptor. The properties of the generated antibodies have been compared after two injections or following a multiple immunisation protocol with the receptor in adjuvant. High titre antisera were readily generated after the short injection protocol and further immunisations did not lead to any change in antibody titers. Analysis of the epitopes recognised using synthetic peptides confirmed previous observations that the immunodominant determinants localise to the amino and the carboxyl terminal part of the extracellular region of the receptor. Antisera from both rabbits contain TSH blocking antibody as assessed by inhibition of TSH mediated cAMP stimulati...Continue Reading

References

Sep 28, 1990·Biochemical and Biophysical Research Communications·J PerretM Parmentier
Feb 1, 1988·Endocrine Reviews·B Rees SmithJ Furmaniak
Jan 1, 1994·Thyroid : Official Journal of the American Thyroid Association·Y NagayamaS Nagataki
Mar 15, 1994·Biochemical and Biophysical Research Communications·S CostagliolaM Ludgate
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·N ShimojoK Tahara
Jan 1, 1996·Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [and] German Diabetes Association·J P Banga

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Citations

Aug 11, 1998·Clinical and Experimental Immunology·S H WangJ P Banga
Feb 21, 2003·Thyroid : Official Journal of the American Thyroid Association·Jennifer JeffreysBernard Rees Smith

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