PMID: 7515261Jun 1, 1994Paper

Characterisation of the differential expression of marker antigens by normal and malignant endometrial epithelium

British Journal of Cancer
E ChatzakiJ G Grudzinskas

Abstract

In order to examine the production of marker proteins, a reproducible method has been established for culturing purified epithelial cells from normal and malignant endometrium. We have examined the differential expression of secretory proteins using immunohistochemistry in frozen tissue sections, immunocytochemistry in cell cultures derived from the same specimens and protein assays on the culture supernatants. Placental protein 14 (PP14) was produced by normal premenopausal epithelium but not by the post-menopausal or malignant endometrial epithelium. In contrast, placental alkaline phosphatase (PLAP) was produced by endometrial cancers and the endometrial adenocarcinoma-derived cell line Ishikawa, but not by the normal endometrial epithelium. Other markers such as CA-125, which was produced by both normal and malignant endometrium but not by the cell line, and human chorionic gonadotrophin (beta-hCG), which was produced by Ishikawa cells but not by any of the fresh tissues, were less cancer specific. Placental alkaline phosphatase is a direct product of endometrial cancers that can be readily assayed in serum using this two-site assay to test its clinical usefulness in monitoring patients at risk for endometrial cancer.

Citations

Jul 5, 2001·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·I R HorowitzS Ramachandran
Apr 22, 2004·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·M Seppälä
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·M SongS Parthasarathy
Dec 15, 2017·Frontiers in Immunology·Juan CuiXiuwen Wang
May 12, 2005·The Journal of Clinical Endocrinology and Metabolism·Michael D MuellerRobert N Taylor
Oct 3, 2017·The International Journal of Biological Markers·Alexandra C KölblUlrich Andergassen
Dec 20, 1996·Molecular and Cellular Endocrinology·S BirkenJ O'Connor

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