PMID: 7542927Jul 20, 1995Paper

Characterisation of the divalent cation channels of the hepatocyte plasma membrane receptor-activated Ca2+ inflow system using lanthanide ions

Biochimica Et Biophysica Acta
K C Fernando, Greg J Barritt

Abstract

The ability of Gd3+ to inhibit vasopressin-stimulated Ca2+ inflow to hepatocytes was compared with its effect on Mn2+ inflow. In the absence of Gd3+, the stimulation of Mn2+ inflow by vasopressin increased with increasing pH of the extracellular medium. Maximal inhibition of vasopressin-stimulated Ca2+ and Mn2+ inflow by saturating concentrations of Gd3+ was 70 and 30%, respectively. Gd3+ also inhibited thapsigargin-stimulated Ca2+ and Mn2+ inflow with maximal inhibition of 70 and 40%, respectively. It is concluded that vasopressin and thapsigargin each activate two types of Ca2+ inflow processes, one which is sensitive and one which is insensitive to lanthanides. The nature of the pore of the lanthanide-sensitive Ca2+ channel was investigated further using different lanthanides as inhibitors. Tm3+, Gd3+, Eu3+, Nd3+ and La3+ each inhibited vasopressin-stimulated Ca2+ and Mn2+ inflow but had no effect on Ca2+ inflow in the absence of an agonist, or on vasopressin-stimulated release of Ca2+ from intracellular stores. Maximal inhibition of vasopressin-stimulated Ca2+ inflow in the presence of a saturating concentration of each lanthanide ranged from 70-90%. An equation which describes a 1:1 interaction of the lanthanide with a put...Continue Reading

References

May 15, 1992·The Biochemical Journal·J LlopisS Orrenius
Jan 23, 1992·Nature·E Neher
Dec 1, 1991·The American Journal of Physiology·C E Bear, C H Li
Aug 1, 1990·FEBS Letters·T J Rink
Feb 1, 1986·Cell Calcium·J W Putney
Jun 15, 1989·The Biochemical Journal·A L SavageB R Martin
Jun 30, 1988·Biochemical and Biophysical Research Communications·J G AltinF L Bygrave
Oct 1, 1987·Journal of Cellular Physiology·A W SasakiR C Wagner
Aug 1, 1985·Quarterly Reviews of Biophysics·I M Klotz
Jun 1, 1972·The Journal of Physiology·M Claret, J L Mazet
May 1, 1984·The American Journal of Physiology·C R Scheid, F S Fay
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C FasolatoR Penner
Nov 11, 1993·Nature·G Yellen
Jun 1, 1993·The Journal of Physiology·M Hoth, R Penner
Oct 1, 1993·Endocrine Reviews·J W Putney, G S Bird
Jan 28, 1993·Nature·M J Berridge
Oct 22, 1993·Cell·J W Putney, G S Bird

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Citations

Feb 6, 2003·Archives of Pharmacal Research·Hyun-Sook KimDong-Soon Im
Dec 22, 1999·British Journal of Pharmacology·P J BalesP D Marley
Sep 16, 2003·Biochimica Et Biophysica Acta·Nancy E RawsonMark I Friedman
Feb 23, 2008·Biochimica Et Biophysica Acta·Gregory J BarrittGrigori Y Rychkov
Aug 14, 2018·Chemical Communications : Chem Comm·Emilie MathieuK Eszter Borbas
Mar 30, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M R ChoD E Golan
Oct 23, 2001·American Journal of Physiology. Heart and Circulatory Physiology·M J DavisE Wilson

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