Characterisation of the Fusarium graminearum-Wheat Floral Interaction.

Journal of Pathogens
Neil BrownKim E Hammond-Kosack

Abstract

Fusarium Ear Blight is a destructive fungal disease of cereals including wheat and can contaminate the crop with various trichothecene mycotoxins. This investigation has produced a new β-glucuronidase (GUS) reporter strain that facilitates the quick and easy assessment of plant infection. The constitutively expressed gpdA:GUS strain of Fusarium graminearum was used to quantify the overall colonisation pattern. Histochemical and biochemical approaches confirmed, in susceptible wheat ear infections, the presence of a substantial phase of symptomless fungal growth. Separate analyses demonstrated that there was a reduction in the quantity of physiologically active hyphae as the wheat ear infection proceeded. A simplified linear system of rachis infection was then utilised to evaluate the expression of several TRI genes by RT-qPCR. Fungal gene expression at the advancing front of symptomless infection was compared with the origin of infection in the rachis. This revealed that TRI gene expression was maximal at the advancing front and supports the hypothesis that the mycotoxin deoxynivalenol plays a role in inhibiting plant defences in advance of the invading intercellular hyphae. This study has also demonstrated that there are trans...Continue Reading

References

Jul 1, 1995·Molecular Plant-microbe Interactions : MPMI·R H ProctorS P McCormick
Dec 21, 2002·The Plant Journal : for Cell and Molecular Biology·Martin UrbanKim Hammond-Kosack
Nov 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Carin JansenFrank J Maier
Nov 10, 2005·Journal of Agricultural and Food Chemistry·Rex B DyerDaren W Brown
Sep 11, 2007·Bioscience, Biotechnology, and Biochemistry·Makoto KimuraMakoto Fujimura
May 1, 2008·Journal of Agricultural and Food Chemistry·Felicia Wu, Gary P Munkvold
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Sep 1, 2003·Molecular Plant Pathology·Martin UrbanKim Hammond-Kosack
Dec 1, 1997·Plant Disease·Marcia McMullenDale Gallenberg

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Citations

Oct 16, 2015·FEMS Microbiology Reviews·Neil A BrownKim E Hammond-Kosack
Oct 7, 2015·Trends in Plant Science·Helen C Brewer, Kim E Hammond-Kosack
Apr 5, 2012·Molecular Plant Pathology·Ralph DeanGary D Foster
Nov 5, 2016·Molecular Plant-microbe Interactions : MPMI·Silvio TundoRenato D'Ovidio
Apr 16, 2017·Molecular Plant Pathology·Kemal Kazan, Donald M Gardiner
Feb 25, 2017·BMC Genomics·Benoit LaurentMarie Foulongne-Oriol
Jul 1, 2020·Frontiers in Plant Science·Francis FabreLudovic Bonhomme
Nov 7, 2019·Toxins·Nora A ForoudHermann Bürstmayr
Apr 21, 2021·Journal of Experimental Botany·Ana K Machado WoodKostya Kanyuka

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Methods Mentioned

BETA
electron microscopy
transgenic
ELISA
dissection
PCR
reverse transcription quantitative PCR
light microscopy
confocal microscopy
phosphotransferase

Software Mentioned

CIMMYT

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