Characterisation of the gene family encoding acetoacetyl-CoA thiolase in Arabidopsis

Functional Plant Biology : FPB
Iván AhumadaAlbert Boronat

Abstract

Thiolases are ubiquitous enzymes involved in many essential biochemical processes. Biosynthetic thiolases, also known as acetoacetyl-CoA thiolases (AACT), catalyse a reversible Claisen-type condensation of two acetyl-CoA molecules to form acetoacetyl-CoA. Here, we report the characterisation of two genes from Arabidopsis thaliana L., ACT1 and ACT2, which encode two closely related AACT isoforms (AACT1 and AACT2, respectively). Transient expression of constructs encoding AACT1 and AACT2 fused to GFP revealed that the two proteins show a different subcellular localisation. While AACT1 is found in peroxisomes, AACT2 localises in the cytosol and the nucleus. The peroxisomal localisation of AACT1 depends on the presence of a C-terminal peroxisomal targeting sequence (PTS1) motif (Ser-Ala-Leu) not previously found in other organisms. ACT1 and ACT2 genes are also differentially expressed. Whereas ACT2 is expressed at relatively high level in all plant tissues, the expression of ACT1 is restricted to roots and inflorescences and its transcript is present at very low levels. The obtained results are in agreement with the involvement of AACT2 in catalysing the first step of the mevalonate pathway. The metabolic function of AACT1 is not c...Continue Reading

References

Aug 1, 1992·Journal of Molecular Evolution·J C IgualJ E Pérez-Ortín
Aug 1, 1995·Plant Molecular Biology·G Bojorquez, M A Gómez-Lim
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·M EnjutoA Boronat
Mar 29, 1996·The Journal of Biological Chemistry·N CunilleraA Ferrer
Sep 1, 1996·Plant Physiology·J A FrugoliC R McClung
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·L L Madison, G W Huisman
May 20, 1999·Critical Reviews in Biochemistry and Molecular Biology·T J BachK U Vollack
Apr 15, 2000·Journal of Molecular Biology·Y Modis, R K Wierenga
May 12, 2000·European Journal of Biochemistry·V D AntonenkovG P Mannaerts
Jan 5, 2002·Progress in Lipid Research·Ian A Graham, Peter J Eastmond
Mar 14, 2002·Plant Physiology·Yuehui HeSusheng Gan
Nov 13, 2003·Current Opinion in Plant Biology·Marcio C Silva-Filho
Dec 19, 2003·Protein Expression and Purification·Anke C SchiedelJames H Dyer
May 14, 2004·Plant Physiology·M Cruz CastilloJosé León
Jun 28, 2005·Journal of Molecular Evolution·Juli PeretóDavid Moreira
Nov 18, 2005·Progress in Lipid Research·Florence BouvierBilal Camara
Nov 26, 2005·Archives of Biochemistry and Biophysics·Andréa HemmerlinThomas J Bach
Dec 1, 1992·Plant Physiology·N Raikhel
Jul 1, 1985·Evolution; International Journal of Organic Evolution·Joseph Felsenstein

❮ Previous
Next ❯

Citations

Mar 31, 2011·Plant Biotechnology Journal·Kimberley TilbrookStevens M Brumbley
Sep 13, 2011·Journal of Experimental Botany·Gabriela SotoNicolás Daniel Ayub
Mar 5, 2013·Annual Review of Plant Biology·Eva VranováWilhelm Gruissem
Feb 4, 2012·The Arabidopsis Book·Dorothea Tholl, Sungbeom Lee
Jul 30, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yeon Bok KimSang Un Park
Nov 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Matthew E BergmanMichael A Phillips
Feb 7, 2013·The New Phytologist·Natalia DudarevaIan Kaplan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.