PMID: 9188168Jan 1, 1997Paper

Characterisation of the low affinity interaction between rat cell adhesion molecules CD2 and CD48 by analytical ultracentrifugation

European Biophysics Journal : EBJ
H SilkowskiO Byron

Abstract

CD2 is a cell adhesion molecule found on the plasma membrane of T-lymphocytes. Its counter-receptor in rat is the structurally related CD48. This interaction is believed to contribute to the adhesion of T-cells to other cells such as cytotoxic targets and antigen presenting cells. Cell-cell adhesion involves the formation of multiple cell adhesion molecule complexes at the cell surface and if cell-cell de-adhesion is to occur, these complexes need to be disrupted. The affinities of cell adhesion molecule interactions are suggested to be relatively weak to allow this de-adhesion of cell-cell interactions. The CD2/CD48 interaction has been studied using recombinant extracellular proteins and the affinity of the interaction of soluble recombinant rat CD2-CD48 has been determined (at 37 degrees C) using surface plasmon resonance (and shown to be weak), with the dissociation constant Kd = 60-90 microM. The values determined by surface plasmon resonance results could be affected by the immobilisation of the ligand on the chip and any self-association on the chip. We used three different analytical ultracentrifuge procedures which each allowed the interaction to be studied in free solution without the need for an immobilisation medium...Continue Reading

Citations

Aug 13, 1998·Immunological Reviews·S J DavisP A van der Merwe
Feb 23, 2011·Methods : a Companion to Methods in Enzymology·Arthur J Rowe
May 8, 2007·Biophysical Journal·Trushar R PatelArthur J Rowe
Jan 11, 2003·Biotechnology & Genetic Engineering Reviews·Stephen E Harding, Michael P Tombs
May 20, 2000·Biotechnology & Genetic Engineering Reviews·S E HardingI Fiebrig
May 4, 2001·The Journal of Biological Chemistry·N J MacDonaldB K Sim
Apr 25, 2008·Current Protocols in Immunology·Andrea BalboPeter Schuck
Jul 28, 2010·Biochemical Society Transactions·Stephen E Harding, Arthur J Rowe
Jun 17, 1999·Journal of Molecular Biology·C A HolthamP G Stockley
Jan 24, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Ai UshiyamaYoshihisa Kaizuka

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