Characterisation of the Manduca sexta sperm proteome: Genetic novelty underlying sperm composition in Lepidoptera

Insect Biochemistry and Molecular Biology
Emma WhittingtonSteve Dorus

Abstract

The application of mass spectrometry based proteomics to sperm biology has greatly accelerated progress in understanding the molecular composition and function of spermatozoa. To date, these approaches have been largely restricted to model organisms, all of which produce a single sperm morph capable of oocyte fertilisation. Here we apply high-throughput mass spectrometry proteomic analysis to characterise sperm composition in Manduca sexta, the tobacco hornworm moth, which produce heteromorphic sperm, including one fertilisation competent (eupyrene) and one incompetent (apyrene) sperm type. This resulted in the high confidence identification of 896 proteins from a co-mixed sample of both sperm types, of which 167 are encoded by genes with strict one-to-one orthology in Drosophila melanogaster. Importantly, over half (55.1%) of these orthologous proteins have previously been identified in the D. melanogaster sperm proteome and exhibit significant conservation in quantitative protein abundance in sperm between the two species. Despite the complex nature of gene expression across spermatogenic stages, a significant correlation was also observed between sperm protein abundance and testis gene expression. Lepidopteran-specific sperm...Continue Reading

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Citations

Jan 16, 2016·Journal of Proteomics·Helen L BayramDean E Hammond
Jul 5, 2015·Insect Biochemistry and Molecular Biology·Timothy L Karr, James R Walters
Mar 24, 2017·Molecular Biology and Evolution·Alberto VicensSteve Dorus
Sep 30, 2017·Genome Génome / Conseil National De Recherches Canada·Andrew J Mongue, James R Walters
Dec 16, 2018·Molecular & Cellular Proteomics : MCP·Ethan C DegnerSteve Dorus
Apr 12, 2019·Molecular Ecology·Andrew J MongueJames R Walters
Nov 19, 2019·Biological Reviews of the Cambridge Philosophical Society·Scott PitnickSteve Dorus
Jul 8, 2017·BMC Evolutionary Biology·Qian ZhaoMinsheng You
Mar 15, 2019·Molecular & Cellular Proteomics : MCP·Ethan C DegnerSteve Dorus

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