Characterisation of the mantle transcriptome and biomineralisation genes in the blunt-gaper clam, Mya truncata

Marine Genomics
Victoria A SleightM S Clark

Abstract

Members of the Myidae family are ecologically and economically important, but there is currently very little molecular data on these species. The present study sequenced and assembled the mantle transcriptome of Mya truncata from the North West coast of Scotland and identified candidate biomineralisation genes. RNA-Seq reads were assembled to create 20,106 contigs in a de novo transciptome, 18.81% of which were assigned putative functions using BLAST sequence similarity searching (cuttoff E-value 1E-10). The most highly expressed genes were compared to the Antarctic clam (Laternula elliptica) and showed that many of the dominant biological functions (muscle contraction, energy production, biomineralisation) in the mantle were conserved. There were however, differences in the constitutive expression of heat shock proteins, which were possibly due to the M. truncata sampling location being at a relatively low latitude, and hence relatively warm, in terms of the global distribution of the species. Phylogenetic analyses of the Tyrosinase proteins from M. truncata showed a gene expansion which was absent in L. elliptica. The tissue distribution expression patterns of putative biomineralisation genes were investigated using quantitat...Continue Reading

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Citations

Nov 12, 2016·Scientific Reports·Victoria A SleightMelody S Clark
Jul 20, 2019·PloS One·Zhi LiaoHai-Ying Liang
May 14, 2018·Cell Stress & Chaperones·Victoria A SleightMelody S Clark
May 7, 2020·Scientific Reports·João C R CardosoDeborah M Power
Apr 22, 2020·Bioscience, Biotechnology, and Biochemistry·Qi SunZhi Liao
Feb 1, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Jie LiuXiwu Yan

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