Characterisation of the nasal microbiota in granulomatosis with polyangiitis

Annals of the Rheumatic Diseases
Rennie L RheePeter A Merkel

Abstract

Prior studies have suggested a potential link between nasal microbes and granulomatosis with polyangiitis (GPA; Wegener's), but these studies relied on culture-dependent methods. This study comprehensively examined the entire community of nasal microbiota (bacteria and fungi) in participants with GPA compared with healthy controls using deep sequencing methods. 16S rRNA and internal transcribed spacer gene sequencing were performed on nasal microbial DNA isolated from nasal swabs of 60 participants with GPA and 41 healthy controls. Alpha and beta diversity were assessed as well as the relative abundance of the most abundant bacterial and fungal taxa. The effects of covariates including disease activity and immunosuppressive therapies on microbial composition were evaluated. Compared with controls, participants with GPA had a significantly different microbial composition (weighted UniFrac p=0.04) and lower relative abundance of Propionibacterium acnes and Staphylococcus epidermidis (for both, false discovery rate-corrected p=0.02). Disease activity in GPA was associated with a lower abundance of fungal order Malasseziales compared with participants with GPA in remission (p=0.04) and controls (p=0.01). Use of non-glucocorticoid i...Continue Reading

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Aug 3, 2018·Nature Reviews. Rheumatology·Jessica McHugh
Dec 5, 2019·Frontiers in Immunology·Mitsuhiro AkiyamaCornelia M Weyand
Nov 14, 2019·International Journal of Molecular Sciences·Fulvia CeccarelliFabrizio Conti
Jan 26, 2021·Computational and Structural Biotechnology Journal·Andreas KronbichlerJosef Wagner
Nov 14, 2020·Current Opinion in Rheumatology·Shahna Tariq, Alison H Clifford
Jul 3, 2021·International Journal of Molecular Sciences·Antje MüllerPeter Lamprecht
Sep 28, 2021·Frontiers in Cellular and Infection Microbiology·Zhuangzhuang ShiMingzhi Zhang

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