PMID: 8946380Nov 25, 1996Paper

Characterisation of the novel junctions of two minichromosomes of Leishmania major

Molecular and Biochemical Parasitology
G Ortiz, M Segovia

Abstract

The minichromosomes M210 and M230 of Leishmania major are linear amplicons which have an inverted repeat structure. We report here the molecular cloning of the megabasic chromosome region where M210 and M230 recombination points are located. The analysis of this chromosomal region revealed a 374 bp fragment directly repeated 3 times, which was also repeated elsewhere in the genome. DNA fragments containing the novel junctions of the minichromosomes M210 and M230 have been likewise cloned. The nucleotide sequence of these fragments was determined and compared with their corresponding DNAs in the source chromosome. It revealed that M210 contains a central 226 bp non-repeated fragment, whereas the central fragment of M230 was 5 kb long. A 9 bp sequence which was repeated in inverted orientation around the recombination points of M230 was found. M210 showed instead an A + T rich DNA which could be implicated in its generation.

References

Oct 1, 1992·Molecular and Biochemical Parasitology·M NavarroM Segovia
Aug 1, 1991·Molecular and Biochemical Parasitology·C A TrippK Stuart
Feb 1, 1992·Molecular and Biochemical Parasitology·J LiuR Hamers
May 1, 1991·Molecular and Biochemical Parasitology·J H LiuR Hamers
Jul 1, 1987·Molecular and Biochemical Parasitology·R P Bishop, M A Miles
Nov 1, 1994·Molecular and Biochemical Parasitology·M NavarroR Hamers
Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·K SakagamiI Kobayashi

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Citations

Apr 11, 2001·Molecular and Biochemical Parasitology·S M SunkinP J Myler
Jul 28, 2004·Parasitology Today·M Segovia, G Ortiz

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