Characterisation of the proximal airway squamous metaplasia induced by chronic tobacco smoke exposure in spontaneously hypertensive rats.

Respiratory Research
Sarah J BoltonKent E Pinkerton

Abstract

Continuous exposure to tobacco smoke (TS) is a key cause of chronic obstructive pulmonary disease (COPD), a complex multifactorial disease that is difficult to model in rodents. The spontaneously hypertensive (SH) rat exhibits several COPD-associated co-morbidities such as hypertension and increased coagulation. We have investigated whether SH rats are a more appropriate animal paradigm of COPD. SH rats were exposed to TS for 6 hours/day, 3 days/week for 14 weeks, and the lung tissues examined by immunohistochemistry. TS induced a CK13-positive squamous metaplasia in proximal airways, which also stained for Ki67 and p63. We hypothesise that this lesion arises by basal cell proliferation, which differentiates to a squamous cell phenotype. Differences in staining profiles for the functional markers CC10 and surfactant D, but not phospho-p38, indicated loss of ability to function appropriately as secretory cells. Within the parenchyma, there were also differences in the staining profiles for CC10 and surfactant D, indicating a possible attempt to compensate for losses in proximal airways. In human COPD sections, areas of CK13-positive squamous metaplasia showed sporadic p63 staining, suggesting that unlike the rat, this is not a b...Continue Reading

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Citations

Feb 3, 2011·Lung·Lina María Salazar, Ana Milena Herrera
May 26, 2012·TheScientificWorldJournal·Rafael Ramírez-RomeroGustavo Hernández-Vidal
Jun 8, 2010·American Journal of Respiratory Cell and Molecular Biology·Tzipora Goldkorn, Simone Filosto
Dec 20, 2011·American Journal of Respiratory Cell and Molecular Biology·Lei WangKent E Pinkerton
Jun 3, 2014·BMC Pulmonary Medicine·Irene M J EurlingsNiki L Reynaert
Sep 5, 2015·International Forum of Allergy & Rhinology·Tian GaoDe Yun Wang
Jun 21, 2011·Biochemical and Biophysical Research Communications·Benjamin B DavisKent E Pinkerton
Dec 5, 2015·American Journal of Respiratory Cell and Molecular Biology·Laura L HoangKent E Pinkerton
Aug 6, 2013·Journal of Toxicologic Pathology·Hiroyuki TsujiHiroyuki Yoshimura
Dec 25, 2019·Toxicologic Pathology·Ching-Wen WuKent E Pinkerton
Apr 3, 2020·Annals of the American Thoracic Society·George D ThurstonCarl W White
Feb 9, 2021·Chemical Research in Toxicology·Yiying WangXuefei Cao

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Methods Mentioned

BETA
bronchoalveolar
lavage

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