Characterisation of the selective binding of antibiotics vancomycin and teicoplanin by the VanS receptor regulating type A vancomycin resistance in the enterococci

Biochimica Et Biophysica Acta. General Subjects
Charlotte S HughesR Hussain

Abstract

A-type resistance towards "last-line" glycopeptide antibiotic vancomycin in the leading hospital acquired infectious agent, the enterococci, is the most common in the UK. Resistance is regulated by the VanRASA two-component system, comprising the histidine sensor kinase VanSA and the partner response regulator VanRA. The nature of the activating ligand for VanSA has not been identified, therefore this work sought to identify and characterise ligand(s) for VanSA. In vitro approaches were used to screen the structural and activity effects of a range of potential ligands with purified VanSA protein. Of the screened ligands (glycopeptide antibiotics vancomycin and teicoplanin, and peptidoglycan components N-acetylmuramic acid, D-Ala-D-Ala and Ala-D-y-Glu-Lys-D-Ala-D-Ala) only glycopeptide antibiotics vancomycin and teicoplanin were found to bind VanSA with different affinities (vancomycin 70μM; teicoplanin 30 and 170μM), and were proposed to bind via exposed aromatic residues tryptophan and tyrosine. Furthermore, binding of the antibiotics induced quicker, longer-lived phosphorylation states for VanSA, proposing them as activators of type A vancomycin resistance in the enterococci.

References

Nov 15, 1990·Analytical Biochemistry·I H van StokkumF C Groen
Jul 1, 1990·FEMS Microbiology Letters·S Handwerger, A Kolokathis
Nov 1, 1989·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·P E Reynolds
Mar 1, 1986·The Journal of Hospital Infection·F Parenti
Jul 21, 1988·The New England Journal of Medicine·R LeclercqP Courvalin
May 10, 1974·Annals of the New York Academy of Sciences·H R Perkins, M Nieto
May 19, 1971·Journal of the American Chemical Society·G BarthW Voelter
May 1, 1983·The Journal of Antimicrobial Chemotherapy·R PallanzaV Arioli
Sep 1, 1983·Antimicrobial Agents and Chemotherapy·H C Neu, P Labthavikul
Dec 1, 1984·Antimicrobial Agents and Chemotherapy·L VerbistP J De Schepper
Jan 1, 1984·Annual Review of Microbiology·J C Barna, D H Williams
Dec 1, 1981·Environmental Health Perspectives·B Altshuler
Jan 6, 1981·Biochemistry·S W Provencher, J Glöckner
Mar 1, 1995·Antimicrobial Agents and Chemotherapy·D A BeauregardD J Knowles
Jan 1, 1996·The Journal of Infection·M ArthurP Courvalin
Oct 1, 1996·Trends in Microbiology·M ArthurP Courvalin
Jul 1, 1996·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·S EversP Courvalin
Oct 6, 1997·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D N Gerding
May 14, 1998·Chemistry & Biology·P J LollP H Axelsen
Mar 9, 2000·The New England Journal of Medicine·B E Murray
Feb 13, 2001·Antimicrobial Agents and Chemotherapy·M Arthur, R Quintiliani
Jan 25, 2002·Annual Review of Pharmacology and Toxicology·Jeff PootoolalGerard D Wright
Mar 28, 2002·The Journal of Biological Chemistry·Giuliano SiligardiChrisostomos Prodromou
Apr 4, 2003·The New England Journal of Medicine·Soju ChangUNKNOWN Vancomycin-Resistant Staphylococcus aureus Investigative Team
Nov 25, 2003·FEBS Letters·Massoud SaidijamPeter J F Henderson
Apr 6, 2004·Methods in Enzymology·Narasimha Sreerama, Robert W Woody
Feb 11, 2005·Chemical Reviews·Dan KahneChristopher Walsh
Dec 3, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Patrice Courvalin
Dec 3, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Robert C Moellering
Aug 15, 2006·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·K Hiramatsu
Feb 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Arianna RathCharles M Deber
Apr 13, 2010·Nature Chemical Biology·Kalinka KotevaGerard D Wright
Dec 22, 2011·Journal of Synchrotron Radiation·Rohanah HussainGiuliano Siligardi

❮ Previous
Next ❯

Citations

Aug 2, 2018·European Biophysics Journal : EBJ·Aalishaa A AzamMary K Phillips-Jones
Oct 3, 2018·Antimicrobial Agents and Chemotherapy·Grace YimGerard D Wright
Jan 25, 2019·PloS One·Elizabeth C UptonPatrick J Loll
Jan 4, 2020·Protein Science : a Publication of the Protein Society·Peter J Stogios, Alexei Savchenko
Jan 19, 2020·The Journal of Antibiotics·Oleksandr YushchukVictor Fedorenko
Dec 6, 2018·Biochemical Society Transactions·Giuliano SiligardiRohanah Hussain
Mar 9, 2019·Microbiology Spectrum·Keith E Weaver
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Pikyee Ma, Mary K Phillips-Jones
Sep 29, 2021·Chemical Biology & Drug Design·Ashley King, Meghan S Blackledge

❮ Previous
Next ❯

Methods Mentioned

BETA
circular dichroism
fluorescence

Software Mentioned

MSRCD
ImageJ
OriginPro®
CDApps

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Protein Structures (ASM)

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.

Bacterial Protein Structures

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.