Characterisation of the TNF superfamily members CD40L and BAFF in the small-spotted catshark (Scyliorhinus canicula)

Fish & Shellfish Immunology
Ronggai LiChris J Secombes

Abstract

The tumour necrosis factor superfamily (TNFSF) members CD40L and BAFF play critical roles in mammalian B cell survival, proliferation and maturation, however little is known about these key cytokines in the oldest jawed vertebrates, the cartilaginous fishes. Here we report the cloning of CD40L and BAFF orthologues (designated ScCD40L and ScBAFF) in the small-spotted catshark (Scyliorhinus canicula). As predicted both proteins are type II membrane-bound proteins with a TNF homology domain in their extracellular region and both are highly expressed in shark immune tissues. ScCD40L transcript levels correlate with those of TCRα and transcription of both genes is modulated in peripheral blood leukocytes following in vitro stimulation. Although a putative CD40L orthologue was identified in the elephant shark genome the work herein is the first molecular characterisation and transcriptional analysis of CD40L in a cartilaginous fish. ScBAFF was also cloned and its transcription characterised in an attempt to resolve the discrepancies observed between spiny dogfish BAFF and bamboo shark BAFF in previously published studies.

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Citations

Mar 21, 2003·Reproductive Biology and Endocrinology : RB&E·Chiara CateniWilliam C Hamlett
Feb 23, 2013·Fish & Shellfish Immunology·Xin-Jiang LuYu-Hong Shi
Jan 19, 2010·Fish & Shellfish Immunology·Cui-Luan YaoZhi-Yong Wang
Aug 15, 2012·Developmental and Comparative Immunology·Mansourah HusainTiehui Wang
May 25, 2011·Developmental and Comparative Immunology·C J SecombesS Bird
Aug 1, 2006·Developmental and Comparative Immunology·Jennifer I ReddickChris J Secombes
Apr 6, 2004·Developmental and Comparative Immunology·Mark O HuisingB M Lidy Verburg-van Kemenade
Dec 2, 2004·Developmental and Comparative Immunology·Anna GoostreyChristopher J Secombes
Jul 29, 2020·Science China. Life Sciences·Yaoguo LiJun Zou

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