PMID: 377024Apr 17, 1979Paper

Characterisation of Tn1721, a new transposon containing tetracycline resistance genes capable of amplification

Molecular & General Genetics : MGG
R SchmittR Mattes

Abstract

R plasmid pRSD1 contains tetracycline resistance (tet) genes in a 3.55 Mdal-region capable of amplification by forming tandem repeats (Mattes, Burkardt and Schmitt, Molec. gen. Genet., 1979). The repetitious tet element is itself part of a 7.2 Mdal-transposon, named Tn1721, as demonstrated by the following criteria; (i) Tn1721 has been translocated to phage lambda. The resulting hybrid phage lambda tet contains the 7.2 Mdal-insertion to the right of the attachment site, but not continguous with it indicating translocation of the element by non-homologous recombination. In addition, lambda tet has sustained a 3.4 Mdal-deletion adjacent to the insertion. (ii) Further transposition of Tn1721 to the 21.5 Mdal-plasmid R388 resulted in R388::Tn1721 derivatives, two of which were characterised. They contain Tn1721 inserted into different sites but in the same orientation as shown by restriction and heteroduplex analyses. These translocation of Tn1721 were not accompanied by deletions of DNA. (iii) The insertion plasmid pRSD102(R388::Tn1721) has conserved the capacity of the original plasmid pRSD1 to amplify the 3.55 Mdal-tet region. It has been concluded that Tn1721 constitutes a novel transposon encompassing a tet region capable of s...Continue Reading

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Citations

Jan 1, 1981·Molecular & General Genetics : MGG·L J WallaceM H Richmond
Jan 1, 1984·Molecular & General Genetics : MGG·P Rogowsky, R Schmitt
Jan 1, 1983·Molecular & General Genetics : MGG·J Altenbuchner, R Schmitt
Jan 1, 1983·Molecular & General Genetics : MGG·W P DiverR Schmitt
Jan 1, 1985·Molecular & General Genetics : MGG·S E HalfordE A Kirkbride
Jan 1, 1985·Molecular & General Genetics : MGG·A P Jessop, C Clugston
Apr 18, 2002·FEMS Microbiology Letters·Lucia P FerreiraManoel Victor F Lemos
Jul 18, 2009·Nature Reviews. Microbiology·Linus Sandegren, Dan I Andersson
Jul 1, 1980·Antimicrobial Agents and Chemotherapy·S T JørgensenP M Bennett
Nov 1, 1986·Antimicrobial Agents and Chemotherapy·M Arthur, P Courvalin
Feb 1, 1988·Molecular & General Genetics : MGG·E Martinez, F de la Cruz
Jan 5, 2002·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·T M L'Abée-Lund, H Sørum
Nov 1, 1990·Plasmid·J GrinstedR Schmitt
Jan 1, 1980·Scandinavian Journal of Infectious Diseases. Supplementum
Oct 12, 2020·Annals of the New York Academy of Sciences·Antony T VincentSteve J Charette
May 1, 1990·Applied and Environmental Microbiology·J L ArmstrongL A Porteous
Aug 1, 1989·Applied and Environmental Microbiology·J H Paul, A W David
Aug 1, 1983·Journal of Bacteriology·T SpiesF C Riess
Jul 1, 1980·Cell·M P Calos, J H Miller
Mar 1, 1980·Plasmid·B MendezS B Levy

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