Abstract
The aim of this study was to investigate differences in concentrations of vitamin A, transthyretin (TTR) and retinol-binding protein (RBP) between plasma and cerebrospinal fluid (CSF) in dogs. RBP was detected using ELISA, and both RBP and TTR by Western blot analysis after separation on SDS-PAGE. Vitamin A was determined by high performance liquid chromatography. RBP and TTR as well as vitamin A were detected in all samples but at substantially lower concentrations in CSF compared to plasma. RBP in dog plasma showed a similar molecular mass to that of humans, whereas canine TTR had a lower molecular mass. Comparison between plasma and CSF showed that both RBP and TTR were of lower molecular mass in CSF. In CSF, RBP and retinol were present at 10-100-fold lower concentrations compared to plasma. Retinyl esters were present only in minute amounts in 5/17 samples. In conclusion, the CSF of dogs compared to humans is significantly different in terms of both quality and quantity of transport proteins for vitamin A.
References
May 2, 1977·European Journal of Biochemistry·G FexE Thulin
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P N MacDonaldD E Ong
Aug 15, 1970·Nature·U K Laemmli
May 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·A R AldredG Schreiber
Oct 1, 1994·Neuroscience·R H ZetterströmL Olson
Jan 1, 1994·Annual Review of Nutrition·Y Ingenbleek, V Young
May 1, 1996·European Journal of Endocrinology·S JaconiG Siegenthaler
Jul 7, 2000·International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition·F J SchweigertK Bathe
Jul 7, 2000·International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition·F J Schweigert, V Bok
Nov 4, 2000·Biochimica Et Biophysica Acta·H L Monaco
Jan 3, 2001·Microscopy Research and Technique·G SchreiberP Prapunpoj
Jan 3, 2001·Microscopy Research and Technique·A Chodobski, J Szmydynger-Chodobska
Apr 11, 2001·Toxicological Sciences : an Official Journal of the Society of Toxicology·W ZhengW S Blaner
Jul 31, 2001·Transplantation·H TerazakiK Tanaka
Sep 22, 2001·Electrophoresis·R WaitI Miller
Jan 30, 2002·Analytical Biochemistry·Ann Westman-Brinkmalm, Pia Davidsson
Jun 27, 2002·Nutrition and Health·Rune Blomhoff
Oct 29, 2002·Annals of Neurology·Judith E A WarnerKathleen B Digre
Nov 5, 2002·Rapid Communications in Mass Spectrometry : RCM·P DavidssonA Westman-Brinkmalm
Feb 27, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ann B Goodman, Arthur B Pardee
Jul 15, 2003·American Journal of Veterinary Research·Jens RailaFlorian J Schweigert
Citations
Dec 25, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Zhaohe LiShicui Zhang
Jul 28, 2009·Biochemical and Biophysical Research Communications·Camila MatiolloHernán Terenzi
Feb 26, 2008·The Veterinary Journal·Roberta Di Terlizzi, Simon R Platt
Nov 19, 2011·Journal of Veterinary Internal Medicine·H SchaeferJ Raila
Jan 19, 2012·Veterinary Clinical Pathology·Marion PiechottaHans-Otto Hoppen
May 2, 2012·Journal of Proteomics·Fausto ScoppettaElisabetta Chiaradia
Nov 22, 2016·Scientific Reports·Andrea HenzeJulia Bornhorst
Mar 29, 2021·Domestic Animal Endocrinology·R E ShielC T Mooney