Characterisation of two calmodulin-like proteins from the liver fluke, Fasciola hepatica

Biological Chemistry
Sean L RussellDavid J Timson

Abstract

Calmodulin is a calcium ion-sensing signalling protein found in eukaryotics. Two calmodulin-like gene sequences were identified in an EST library from adult liver flukes. One codes for a protein (FhCaM1) homologous to mammalian calmodulins (98% identity), whereas the other protein (FhCaM2) has only 41% identity. These genes were cloned into expression vectors and the recombinant proteins were expressed in Escherichia coli. Gel shift assays showed that both proteins bind to calcium, magnesium and zinc ions. Homology models have been built for both proteins. As expected, FhCaM1 has a highly similar structure to other calmodulins. Although FhCaM2 has a similar fold, its surface charge is higher than FhCaM1. One of the potential metal ion-binding sites has lower metal-ion co-ordination capability, while another has an adjacent lysine residue, both of which may decrease the metal-binding affinity. These differences may reflect a specialised role for FhCaM2 in the liver fluke.

References

Dec 20, 1992·Journal of Molecular Biology·R ChattopadhyayaF A Quiocho
Jun 1, 1992·The American Journal of Tropical Medicine and Hygiene·D R Hopkins
Nov 5, 1988·Journal of Molecular Biology·Y S BabuW J Cook
May 2, 1985·Nature·Y S BabuW J Cook
Jan 29, 1982·Biochemical and Biophysical Research Communications·R Gopalakrishna, W B Anderson
May 11, 1982·Biochemistry·T SasagawaK Titani
Sep 1, 1995·Nature Structural Biology·H KuboniwaA Bax
Nov 1, 1994·Nature Structural Biology·M VandonselaarL T Delbaere
Jul 1, 1995·Australian Veterinary Journal·D J Overend, F L Bowen
Mar 14, 1998·Protein Expression and Purification·N HayashiH Taniguchi
Jul 21, 1999·Molecular and Biochemical Parasitology·A D Ruiz de EguinoF Parra
May 16, 2000·The Veterinary Record·I VangeelF Haesebrouck
Jul 8, 2000·Trends in Cell Biology·D Chin, A R Means
Jan 12, 2002·Biochemical and Biophysical Research Communications·Françoise HaeseleerKrzysztof Palczewski
Feb 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·A M GillettS B Hooper
Sep 5, 2002·The Veterinary Record·Roger Daniel, Sian Mitchell
Nov 26, 2002·The Journal of Biological Chemistry·Zhigang Li, David B Sacks
Jan 25, 2003·European Journal of Biochemistry·Stefan W Vetter, Estelle Leclerc
Jun 26, 2003·Nucleic Acids Research·Ramu ChennaJulie D Thompson
Jun 26, 2003·Nucleic Acids Research·Rodrigo LopezWarren Gish
Jun 5, 2004·FEBS Letters·Stephen R Martin, Peter M Bayley
Oct 21, 2004·The Cochrane Database of Systematic Reviews·N AdhikariM O Meade
Apr 28, 2005·The FEBS Journal·Danny KöhlerMartin Bähler
Sep 13, 2005·Journal of Helminthology·Ian Fairweather
Sep 26, 2006·The Veterinary Record·M A Alvarez-SánchezF A Rojo-Vázquez

Citations

Sep 11, 2013·Parasitology Research·Eun Joo ChungTai-Soon Yong
Aug 19, 2009·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Mark W Robinson, John P Dalton
Jul 24, 2012·International Journal for Parasitology·Catherine M MooreDavid J Timson
Jun 26, 2012·Biochimie·Sean L RussellDavid J Timson
Jan 29, 2016·Parasites & Vectors·Erin M McCammickAaron G Maule
Nov 17, 2010·The FEBS Journal·Hugo FragaAna M Damas
Jul 1, 2008·Molecular and Cellular Biochemistry·Sevvel PathmanathanDavid J Timson

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