Characterisation of vitamin D-related molecules and calcium-sensing receptor in human Fallopian tube during the menstrual cycle and in ectopic pregnancy

Cell and Tissue Research
Bassem RefaatFaizah Ahmed Malibary

Abstract

This is a prospective observational study that measures the expression of vitamin D (VD) metabolising and signalling molecules and Ca2+ sensing receptor (CaSR) in human Fallopian tube (FT) during the menstrual cycle and ectopic pregnancy (EP). Fresh FTs were obtained during total abdominal hysterectomy at the follicular (n = 16) and midluteal (n = 16) phases. Specimens from remote and implantation sites as well as trophoblastic tissues were also freshly collected from each FT with EP (n = 10). All women had normal serum VD and ionised Ca2+. The expression of VD synthesising (CYP27B1) and catalysing (CYP24A1) enzymes, binding protein (VDBP), receptor (VDR), retinoid X receptor (RXR) and CaSR was measured by immunohistochemistry and quantitative RT-PCR. All molecules, except VDBP, were significantly increased (P < 0.05) in midluteal compared with follicular samples. Remote EP sites showed significantly (P < 0.05) lower expression of CYP27B1, CYP24A1, VDR and RXR and a higher expression of VDBP and CaSR (P < 0.05) compared with midluteal samples. Significant differences were observed by immunohistochemistry between implantation and remote sites from EP for all molecules, which were also localised in the trophoblastic tissues. In c...Continue Reading

Citations

Feb 1, 1972·Contraception·J LippesW R Bartholomew
Jan 1, 1993·Gynecologic and Obstetric Investigation·M GoldenbergG Oelsner
Nov 26, 1997·Journal of Reproduction and Fertility·S J DowningH J Leese
May 18, 1999·American Journal of Obstetrics and Gynecology·A CagnacciA Volpe
May 4, 2002·Molecular and Cellular Endocrinology·Haim Breitbart
Jun 1, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Li-Juan XiaoYi-Xun Liu
Apr 4, 2006·Lancet·Khalid S KhanPaul Fa Van Look
Apr 18, 2006·Fertility and Sterility·Maria Elena Dell'AquilaValeria Casavola
May 25, 2006·Journal of Molecular Endocrinology·P ViganòA M Di Blasio
Jan 24, 2007·The Journal of Steroid Biochemistry and Molecular Biology·Steffi BeckerMichael Friedrich
Mar 16, 2007·Annals of the New York Academy of Sciences·Laurentiu M PopescuDragos Cretoiu
Jan 24, 2008·Reproductive Biology and Endocrinology : RB&E·David BarreraLorenza Díaz
Nov 10, 2009·Fertility and Sterility·Amir Hassan ZarnaniMahmood Jeddi-Tehrani
Mar 12, 2011·Journal of Andrology·Francisco J MendozaMariano Rodriguez
Mar 16, 2011·Biology of the Cell·Andreas Schmid, Matthias Salathe
Aug 30, 2011·Best Practice & Research. Clinical Endocrinology & Metabolism·Helen L Henry
Sep 2, 2011·American Journal of Physiology. Cell Physiology·Rose Ellen DixonSean M Ward
Nov 4, 2011·Reproductive Biology and Endocrinology : RB&E·Magdalena Grundmann, Frauke von Versen-Höynck
May 11, 2012·Biology of Reproduction·Cary L DickenGenevieve Neal-Perry
May 24, 2012·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Samira RajaeiAmir Hassan Zarnani
Jul 25, 2012·Human Reproduction·Janelle LukLubna Pal
Aug 9, 2012·American Journal of Physiology. Endocrinology and Metabolism·Rong MaYuping Wang
Feb 23, 2013·Journal of Cardiovascular Pharmacology·Annemarieke E LootIngrid Fleming
Mar 14, 2013·American Journal of Physiology. Endocrinology and Metabolism·Edwin C Y ChowK Sandy Pang
Apr 17, 2013·Journal of Cellular Physiology·V J WoeckelJ P T M Van Leeuwen
Apr 26, 2013·Kidney International·Giovambattista CapassoJohn P Geibel
Nov 23, 2013·American Journal of Obstetrics and Gynecology·Chandrasekhar ThotaNahed Ismail
Nov 26, 2014·Reproductive Biology and Endocrinology : RB&E·Bassem Refaat
Dec 17, 2014·Endocrine·Rachele M PojednicRoger A Fielding
Dec 31, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shankar ThangamaniChang H Kim
Mar 21, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lieqi TangSam X Cheng
Apr 18, 2015·Reproductive Biology and Endocrinology : RB&E·Bassem RefaatWilliam L Ledger
Apr 24, 2015·Science Translational Medicine·Polina L YarovaDaniela Riccardi
Apr 29, 2015·Molecular Reproduction and Development·Maryam TavakoliAmir-Hassan Zarnani
Jun 25, 2015·Experimental Physiology·Irene Lopez-FernandezDaniela Riccardi

Related Concepts

Vitamin A
Retinoid X Receptors
Pathogenic Aspects
Calcium [EPC]
Observational Study
Fallopian Tube Diseases
CT of Abdomen
Pathogenesis
Immunohistochemistry
Serum Vitamin D Measurement

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