Characterisation of vitamin D-related molecules and calcium-sensing receptor in human Fallopian tube during the menstrual cycle and in ectopic pregnancy

Cell and Tissue Research
Bassem RefaatFaizah Ahmed Malibary


This is a prospective observational study that measures the expression of vitamin D (VD) metabolising and signalling molecules and Ca2+ sensing receptor (CaSR) in human Fallopian tube (FT) during the menstrual cycle and ectopic pregnancy (EP). Fresh FTs were obtained during total abdominal hysterectomy at the follicular (n = 16) and midluteal (n = 16) phases. Specimens from remote and implantation sites as well as trophoblastic tissues were also freshly collected from each FT with EP (n = 10). All women had normal serum VD and ionised Ca2+. The expression of VD synthesising (CYP27B1) and catalysing (CYP24A1) enzymes, binding protein (VDBP), receptor (VDR), retinoid X receptor (RXR) and CaSR was measured by immunohistochemistry and quantitative RT-PCR. All molecules, except VDBP, were significantly increased (P < 0.05) in midluteal compared with follicular samples. Remote EP sites showed significantly (P < 0.05) lower expression of CYP27B1, CYP24A1, VDR and RXR and a higher expression of VDBP and CaSR (P < 0.05) compared with midluteal samples. Significant differences were observed by immunohistochemistry between implantation and remote sites from EP for all molecules, which were also localised in the trophoblastic tissues. In c...Continue Reading


Feb 1, 1972·Contraception·J LippesW R Bartholomew
Jan 1, 1993·Gynecologic and Obstetric Investigation·M GoldenbergG Oelsner
Nov 26, 1997·Journal of Reproduction and Fertility·S J DowningH J Leese
May 18, 1999·American Journal of Obstetrics and Gynecology·A CagnacciA Volpe
May 4, 2002·Molecular and Cellular Endocrinology·Haim Breitbart
Jun 1, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Li-Juan XiaoYi-Xun Liu
Apr 4, 2006·Lancet·Khalid S KhanPaul Fa Van Look
Apr 18, 2006·Fertility and Sterility·Maria Elena Dell'AquilaValeria Casavola
May 25, 2006·Journal of Molecular Endocrinology·P ViganòA M Di Blasio
Jan 24, 2007·The Journal of Steroid Biochemistry and Molecular Biology·Steffi BeckerMichael Friedrich
Mar 16, 2007·Annals of the New York Academy of Sciences·Laurentiu M PopescuDragos Cretoiu
Jan 24, 2008·Reproductive Biology and Endocrinology : RB&E·David BarreraLorenza Díaz
Nov 10, 2009·Fertility and Sterility·Amir Hassan ZarnaniMahmood Jeddi-Tehrani
Mar 12, 2011·Journal of Andrology·Francisco J MendozaMariano Rodriguez
Mar 16, 2011·Biology of the Cell·Andreas Schmid, Matthias Salathe
Aug 30, 2011·Best Practice & Research. Clinical Endocrinology & Metabolism·Helen L Henry
Sep 2, 2011·American Journal of Physiology. Cell Physiology·Rose Ellen DixonSean M Ward
Nov 4, 2011·Reproductive Biology and Endocrinology : RB&E·Magdalena Grundmann, Frauke von Versen-Höynck
May 11, 2012·Biology of Reproduction·Cary L DickenGenevieve Neal-Perry
May 24, 2012·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Samira RajaeiAmir Hassan Zarnani
Jul 25, 2012·Human Reproduction·Janelle LukLubna Pal
Aug 9, 2012·American Journal of Physiology. Endocrinology and Metabolism·Rong MaYuping Wang
Feb 23, 2013·Journal of Cardiovascular Pharmacology·Annemarieke E LootIngrid Fleming
Mar 14, 2013·American Journal of Physiology. Endocrinology and Metabolism·Edwin C Y ChowK Sandy Pang
Apr 17, 2013·Journal of Cellular Physiology·V J WoeckelJ P T M Van Leeuwen
Apr 26, 2013·Kidney International·Giovambattista CapassoJohn P Geibel
Nov 23, 2013·American Journal of Obstetrics and Gynecology·Chandrasekhar ThotaNahed Ismail
Nov 26, 2014·Reproductive Biology and Endocrinology : RB&E·Bassem Refaat
Dec 17, 2014·Endocrine·Rachele M PojednicRoger A Fielding
Dec 31, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shankar ThangamaniChang H Kim
Mar 21, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lieqi TangSam X Cheng
Apr 18, 2015·Reproductive Biology and Endocrinology : RB&E·Bassem RefaatWilliam L Ledger
Apr 24, 2015·Science Translational Medicine·Polina L YarovaDaniela Riccardi
Apr 29, 2015·Molecular Reproduction and Development·Maryam TavakoliAmir-Hassan Zarnani
Jun 25, 2015·Experimental Physiology·Irene Lopez-FernandezDaniela Riccardi

Related Concepts

Vitamin A
Retinoid X Receptors
Pathogenic Aspects
Calcium [EPC]
Observational Study
Fallopian Tube Diseases
CT of Abdomen
Serum Vitamin D Measurement

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.