Characterising PvRBSA: an exclusive protein from Plasmodium species infecting reticulocytes

Parasites & Vectors
Darwin A Moreno-PérezManuel A Patarroyo

Abstract

Plasmodium vivax uses multiple ligand-receptor interactions for preferential invasion of human reticulocytes. Several of these ligands have been identified by in silico approaches based on the role displayed by their orthologs in other Plasmodium species during initial adhesion or invasion. However, the cell adhesion role of proteins that are exclusive to species that specifically invade reticulocytes (as P. vivax and P. cynomolgi) has not been evaluated to date. This study aimed to characterise an antigen shared between Plasmodium species that preferentially infect reticulocytes with a focus on assessing its binding activity to target cells. An in silico analysis was performed using P. vivax proteome data to identify and characterise one antigen shared between P. vivax and P. cynomolgi. This led to identification of the pvrbsa gene present in the P. vivax VCG-I strain genome. This gene is transcribed in mature schizonts and encodes a protein located on the parasite surface. rPvRBSA was antigenic and capable of binding to a population of reticulocytes with a different Duffy phenotype. Interestingly, the molecule showed a higher percentage of binding to immature human reticulocytes (CD71hi). This study describes for the first ti...Continue Reading

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Citations

Feb 10, 2018·Malaria Journal·Darwin Andrés Moreno-PérezManuel Alfonso Patarroyo
Sep 27, 2018·Frontiers in Genetics·Paola Andrea Camargo-AyalaManuel A Patarroyo
Aug 22, 2018·Malaria Journal·Maritza BermúdezManuel Alfonso Patarroyo
Oct 27, 2018·Current Opinion in Microbiology·Usheer KanjeeManoj T Duraisingh
May 16, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Eugenia LoGiacomo Maria Paganotti
Aug 7, 2021·Pathogens·Jing-Wen HangBenoit Malleret

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Datasets Mentioned

BETA
KY349105

Methods Mentioned

BETA
protein assay
PCR
ELISA
Flow cytometry

Software Mentioned

FlowjoV10
GradhPad
HMMTOP
Phobius
ClustalW ( NPS )
ANTHEPROT
SignalP
BaCelLo
FragAnchor
TMHMM

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