Characteristic and Functional Study of Intersex , a Gene Related to Female Fertility in Bemisia tabaci

Frontiers in Physiology
Yating LiuYoujun Zhang

Abstract

The intersex (ix) gene acts in concert with doublesex (dsx) at the end of the sex determination hierarchy to control somatic sexual differentiation in Drosophila melanogaster. Here, we report the Drosophila ix homolog in Bemisia tabaci (Btix) with differential splicing events. Four isoforms were found in B. tabaci adults, including two sex-specific transcripts (Btix F and Btix M ). Knockdown of Btix had no measurable effects on female morphological phenotypes but reduced the expression of the vitellogenin gene and resulted in the production of significantly fewer eggs, a lower eclosion rate, and a shorter body size of female progeny in comparison with control females. These results increase our understanding of the genes underlying sex determination in B. tabaci and reveal a potential target for RNA interference-based pest management.

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Datasets Mentioned

BETA
QAB02863.1

Methods Mentioned

BETA
transgenic
PCRs
electrophoresis
PCR

Software Mentioned

MEGA
SPSS
Basic Local Alignment Search Tool ( BLAST )
ClustalW
ExPASy ProtParam
ExPASy BOXSHADE
ORF Finder

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