Characteristics and comparative performance of direct culture, direct PCR and enumeration methods for detection and quantification of Campylobacter spp. in broiler caeca

Veterinary Microbiology
J RodgersF Clifton-Hadley

Abstract

Detection and enumeration of Campylobacter spp. in broiler chicken flocks are key components of research and surveillance studies aimed at reducing Campylobacter infections in people. Direct culture of caecal contents onto selective agar is the typical method used to confirm flock colonisation. Modified charcoal cefoperazone deoxycholate agar (mCCDA) is commonly used for this method, although alternative selective media have been used. Additionally, PCR methods to detect Campylobacter DNA from caecal contents may provide a rapid alternative. However comparative performance data for these methods is limited and therefore required to ensure optimal detection methods for this sample type. In this study, 306 broiler caeca were tested for Campylobacter using direct culture on mCCDA, Skirrows and Preston agars and two real-time PCR methods, one specific for mapA/ceuE regions and another for the flaA gene region. Additionally, the suitability of spread plating and spiral plating methods for enumeration of Campylobacter and the impact of sample storage were assessed. This study confirmed modified CCDA as an optimal media for detection of Campylobacter in broiler caeca. It was significantly more sensitive than Skirrows or Preston agars....Continue Reading

References

Jan 1, 1992·International Journal of Food Microbiology·E BerndtsonA Engvall
Nov 6, 2004·Journal of Clinical Microbiology·Marianne LundMogens Madsen
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Citations

Mar 17, 2015·Journal of Microbiological Methods·Mathilde H JosefsenJeffrey Hoorfar
Jun 11, 2021·Frontiers in Veterinary Science·Robert Valeris-ChacinRandall S Singer

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