PMID: 9425247Mar 14, 1998Paper

Characteristics and DNA-sequence of a cryptic haloalkanoic acid dehalogenase from Agrobacterium tumefaciens RS5

Current Microbiology
R KöhlerF R Schmidt

Abstract

Agrobacterium tumefaciens RS5 harbors two different hydrolytic haloalkanoic acid dehalogenase genes, one coding for a nonstereospecific enzyme (DhlS5II) and a second for a cryptic L-isomer-specific dehalogenase (DhlS5I). The latter gene was cloned and expressed in Escherichia coli. Biochemical characterization and sequence analysis of dhlS5I shows its membership to the class of the L-isomer-specific hydrolytic dehalogenases. Highest homology of 72% was found to the dehalogenase LdexYL from Pseudomonas sp. YL. Both enzymes share an unusual high temperature optimum of 65 degrees C. Controlled by a vector promoter, high specific dehalogenase activities up to 32 U mg-1 protein were obtained in E. coli, and putatively, owing to its own sigma70-dependent promoter, a constitutive low-level expression of dhlS5I of 0.4 U mg-1 protein was measured.

Citations

Aug 7, 2003·Applied and Environmental Microbiology·Julian R Marchesi, Andrew J Weightman
Apr 10, 2013·Biotechnology and Bioengineering·Xianbin SuJimmy S H Tsang
May 8, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Thiau-Fu AngThean Chor Leow
Jan 17, 2016·Applied and Environmental Microbiology·Tomas BuryskaZbynek Prokop
May 26, 2016·Chembiochem : a European Journal of Chemical Biology·Johannes GrossMélanie Hall

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