Characteristics and EGFP expression of goat mammary gland epithelial cells

Reproduction in Domestic Animals = Zuchthygiene
Y M ZhengY Zhang

Abstract

The aims of this study were (i) to establish a goat mammary gland epithelial (GMGE) cell line, and (ii) to determine if these GMGE cells could be maintained long-term in culture by continuous subculturing following transfection with a reporter gene, enhanced green fluorescence protein (EGFP). Primary culture of GMGE cells was achieved by outgrowth of migrating cells from the fragments of the mammary gland tissue of a lactating goat. The passage 16 GMGE cells were transfected with EGFP gene using lipofection. The expression of Cell keratins of epithelial cells in GMGE cells was test by immunofluorescence. Βeta-Casein gene mRNA was test for GMGE cells by RT-PCR. The results showed that when grown at low density on a plastic substratum, the GMGE cells formed islands, and when grown to confluency, the cells formed a monolayer and aggregated with the characteristic cobble-stone morphology of epithelial cells. GMGE cells could form dome-like structure which looked like nipple, and the lumen-like structures formed among the cells. Several blister-like structures appeared in the appearance of the cells. The GMGE cells contained different cell types, majority of the cells were short shuttle-like or polygon which were beehive-like. A par...Continue Reading

References

Jul 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·C A GibsonC W Welsch
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K G DanielsonD Medina
May 1, 1980·In Vitro·M StampferA J Hackett
Mar 1, 1996·In Vitro Cellular & Developmental Biology. Animal·B ZavizionI Politis
May 20, 1998·In Vitro Cellular & Developmental Biology. Animal·N IlanM Shani
Aug 6, 1998·Biology of the Cell·M DüchlerK Schellander
Jan 16, 1999·Biotechnology Annual Review·J JänneV P Korhonen
Feb 26, 2000·In Vitro Cellular & Developmental Biology. Animal·A G PantschenkoT J Yang
Oct 11, 2001·Veterinary Research·L Mselli-LakhalY Chebloune
Jan 5, 2002·Theriogenology·M B Wheeler, E M Walters
Oct 9, 2002·The Journal of Dairy Research·Michael T RoseYoshiaki Obara
Nov 7, 2002·In Vitro Cellular & Developmental Biology. Animal·Tania German, Itamar Barash
Dec 4, 2003·Animal Reproduction Science·Heiner Niemann, Wilfried A Kues

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Citations

Nov 28, 2012·In Vitro Cellular & Developmental Biology. Animal·Ramakant KaushikManmohan Singh Chauhan
Sep 13, 2014·In Vitro Cellular & Developmental Biology. Animal·Sonja Prpar MihevcPeter Dovc
Jun 1, 2011·Expert Opinion on Biological Therapy·Petra A B KlemmtBernd Groner
Feb 21, 2018·Anatomia, histologia, embryologia·N SaipinR Rungsiwiwut
Nov 12, 2014·Biotechnology and Applied Biochemistry·Kai-Hung WangTsung-Cheng Kuo
Jan 2, 2014·Molecular Biology Reports·Liqi ZhuQian Yang

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