Characteristics and prevalence within serogroup O4 of a J96-like clonal group of uropathogenic Escherichia coli O4:H5 containing the class I and class III alleles of papG.

Infection and Immunity
J R JohnsonJ N Maslow

Abstract

The recent discovery of a geographically dispersed clonal group of Escherichia coli O4:H5 that includes prototypic uropathogenic strain J96 prompted us to determine the prevalence of J96-like strains within serogroup O4 and to further assess the characteristics of such strains. We used O:K:H;F serotyping, PCR-based genomic fingerprinting, pulsed-field gel electrophoresis (PFGE), multilocus enzyme electrophoresis (MLEE), and PCR detection of the three papG alleles and of the cytotoxic necrotizing factor 1 (cnf1) and aerobactin (aer) gene sequences to characterize the 15 O4 strains among 336 E. coli isolates from three clinical collections (187 from mixed-source bacteremia, 75 from urosepsis, and 74 from acute cystitis). J96-like strains constituted approximately half of the O4 strains, or 2% of the total population. In contrast to other O4 strains, the J96-like strains characteristically exhibited specific group III capsular antigens, the H5 flagellar and F13 fimbrial antigens, a distinctive PCR genomic fingerprint, the class III papG allele (plus, in 50% of strains, the enigmatic class I papG allele), and cnf1 but lacked aer. A subset of these strains was remarkably homogeneous with respect to all these characteristics and exhi...Continue Reading

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Citations

Jul 17, 2020·Journal of Clinical Microbiology·Anna Maria Malberg TetzschnerFlemming Scheutz
Mar 20, 2001·Infection and Immunity·J R JohnsonT T O'Bryan
Sep 19, 2000·Infection and Immunity·L J Melkerson-WatsonC A Bloch
Feb 17, 2001·Infection and Immunity·J R JohnsonP Delavari
Dec 23, 2004·Letters in Applied Microbiology·F L NowrouzianI Adlerberth
Sep 27, 2007·Zoonoses and Public Health·R SuraR A French
Sep 14, 2002·Infection and Immunity·Anne-Beatrice Blanc-PotardMarie-Francoise Bernet-Camard
Nov 1, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ming-Cheng WangJeng-Jong Huang
Feb 3, 2006·International Journal of Clinical Practice·M ArisoyN Akar
Dec 1, 2001·Journal of the American Society of Nephrology : JASN·John H WuDaniel F Balkovetz
Nov 20, 2002·Infection and Immunity·James R Johnson
Aug 30, 2001·Journal of Clinical Microbiology·M Rivera-Betancourt, J E Keen
Jan 24, 2004·Microbes and Infection·Brian K WadeDaniel F Balkovetz
Dec 9, 2021·Microbiology Spectrum·Tatsuya KobayashiKyoji Moriya

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