Characteristics for phage-encoded cell wall hydrolase of LysSAP27 to reduce staphylococcal food poisoning.

Food Science and Biotechnology
Do-Won ParkJong-Hyun Park

Abstract

To reduce staphylococcal food poisoning (SFP), a phage-encoded cell wall hydrolase called endolysin has emerged as an attractive antibacterial agent. In this study, the Staphylococcus aureus infecting phage vB_SauS-SAP27 (ϕSAP27) was isolated from sewage and characterized morphologically and genetically. ϕSAP27 was identified as Siphoviridae temperate phage, with a genome of 43 kbp. A ϕSAP27 endolysin named LysSAP27 was produced recombinantly in Escherichia coli. LysSAP27 exhibited the highest activity at neutral pH and a temperature of 30 °C, and its lytic activity was upregulated by calcium ions. Following optimization of the enzymatic conditions, LysSAP27 was applied to S. aureus-contaminated milk. Treatment with 2 μM LysSAP27 led to a significant bactericidal effect, corresponding to a reduction in bacterial titer by 2.8 log CFU/mL within 1 h and 3.4 log CFU/mL within 2 h. Therefore, LysSAP27 could be used as an effective antimicrobial agent to prevent SFP in food. The online version contains supplementary material available at 10.1007/s10068-021-00910-2.

References

Oct 24, 2006·FEMS Microbiology Letters·David M DonovanJuli Foster-Frey
Nov 8, 2008·Bioinformatics·Tim CarverJulian Parkhill
Dec 11, 2008·Methods in Molecular Biology·Hans-W Ackermann
Dec 11, 2008·Methods in Molecular Biology·Elizabeth Kutter
Aug 15, 2009·Nature Reviews. Microbiology·Henry F Chambers, Frank R Deleo
Apr 3, 2010·Advances in Applied Microbiology·Paul Hyman, Stephen T Abedon
Oct 28, 2010·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Adi Stern, Rotem Sorek
Oct 13, 2011·Molecular Systems Biology·Fabian SieversDesmond G Higgins
Oct 2, 2012·Applied and Environmental Microbiology·Diana GutiérrezPilar García
Feb 15, 2013·Journal of Virology·Hugo OliveiraLeon D Kluskens
Mar 20, 2014·Bioinformatics·Torsten Seemann
May 8, 2014·BioMed Research International·Jhalka KadariyaDipendra Thapaliya
May 18, 2016·FEMS Microbiology Letters·Hongjun NaSangryeol Ryu
Jul 15, 2016·Microbiology and Molecular Biology Reviews : MMBR·Stineke van HouteEdze R Westra
Mar 25, 2019·Applied and Environmental Microbiology·Abraham G MollerTimothy D Read

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.