Characteristics investigation on biofilm formation and biodegradation activities of Pseudomonas aeruginosa strain ISJ14 colonizing low density polyethylene (LDPE) surface

Heliyon
Kartikey Kumar Gupta, Deepa Devi

Abstract

The accelerated population and industrial development have caused an extensive increase in the use of plastic products. Since polyethylene degrades slowly generating poisonous compounds, therefore, elimination of plastic from the environment is the prerequisite requirement today. Biodegradation of plastics seems to be a convenient and effective method to curb this problem. In view of this, the present study focuses on LDPE degradation capability of bacterial strain Pseudomonas aeruginosa ISJ14 (Accession No. MG554742) isolated from waste dump sites. Further, the stability of 16S rDNA of the isolate was determined by applying bioinformatics tools. For biodegradation studies, the polyethylene films were incubated with the culture of P. aeruginosa ISJ14 in two different growth medium namely Bushnell Hass broth (BHM) and Minimal Salt medium (MSM) for 60 days at 37 °C on 180 rpm. In addition, hydrophobicity and viability of bacterial isolate along with quantification of total protein content was also done. The microbial degradation was confirmed by surface modification and formation of fissures on polyethylene surface along with the variation in the intensity of functional groups as well as an increase in the carbonyl index using fi...Continue Reading

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Citations

Nov 17, 2020·The Science of the Total Environment·Ayodeji AmobonyeSanthosh Pillai
May 26, 2021·Biodegradation·Rangasamy Kavitha, Vembhu Bhuvaneswari

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Datasets Mentioned

BETA
MG554742.1

Methods Mentioned

BETA
PCR
chemical treatment

Software Mentioned

Mfold
BLAST
MEGA X
NEB cutter
MEGA
CLUSTRAL

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