Jul 1, 1977

Characteristics of a human cell line transformed by DNA from human adenovirus type 5

The Journal of General Virology
F L GrahamR Nairn

Abstract

Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA. The transformed cells (designated 293 cells) exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumour antigen. Analysis of the polypeptides synthesized in the 293 cells by labelling with 35S-methionine and SDS PAGE showed a variable pattern of synthesis, different in a number of respects from that seen in otheruman cells. On labelling the surface of cells by lactoperoxidase catalysed radio-iodination, the absence of a labelled polypeptide analogous to the 250 K (LETS) glycoprotein was noted. Hybridization of labelled cellular RNA with restriction fragments of adenovirus type 5 DNA indicated transcription of a portion of the adenovirus genome at the conventional left hand end.

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Mentioned in this Paper

Peptide Biosynthesis
Viral Proteins
DNA, Viral
Neoplasm Transplantation
Tumor Lysis Syndrome
Neoglycoproteins
Cell Division Phases
Antigens, Viral
Tumor Antigens
Neoplastic Cell Transformation

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