PMID: 16512421Mar 4, 2006Paper

Characteristics of a protein error in determination of serum protein in the presence of inorganic salt

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Yuji Suzuki

Abstract

There is a possibility that the color development of the dye-binding method based on a protein error of a pH indicator is affected by the coexisting inorganic salt. Thus, the author theoretically and experimentally investigated the effect of the inorganic salt on the protein error. In a theoretical analysis, the anion of an inorganic salt, like the dissociated dye and buffer anions, was assumed to react with the protein, forming a colorless anion-protein complex. The calculated results were compared with those obtained by experiments using three pH indicators and various kinds of inorganic salts. The calculated results obtained are as follows: (1) The color development decreases with increasing the concentration of the inorganic salt and the equilibrium constant of the reaction between the inorganic salt and protein; (2) The rate of the absorbance decrease is larger for a lower concentration of the inorganic salt than for a higher one; (3) The larger is the equilibrium constant, the larger is the absorbance decrease. The absorbance decrease was caused by the anion, and was increased by increasing the anion concentration. The magnitude of the effect of the anion was iodide > bromide > chloride, which was associated with their io...Continue Reading

References

Jan 1, 1971·Clinica Chimica Acta; International Journal of Clinical Chemistry·B T DoumasH G Biggs
Nov 11, 1999·Clinica Chimica Acta; International Journal of Clinical Chemistry·Y MuramotoT Irino
Oct 1, 1953·American Journal of Clinical Pathology·J S BRACKEN, I M KLOTZ
Nov 1, 1959·Clinica Chimica Acta; International Journal of Clinical Chemistry·P G SCHEURLEN
Oct 14, 2004·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yuji Suzuki

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