PMID: 11900838Mar 20, 2002Paper

Characteristics of a respiratory syncytial virus persistently infected macrophage-like culture

Virus Research
Rosa Elena SarmientoB Gomez

Abstract

A persistently infected culture obtained from immortalized murine macrophage-like cells, which survived respiratory syncytial virus (RSV) infection at multiplicity of one, was established and characterized. The presence of RSV through the passages was confirmed and monitored by (a) detection of infectious virus by TCID(50)/ml, (b) defective particles by viral infectivity interference and buoyant density determinations, (c) cell surface antigen by indirect immunofluorescence and FACS, and (d) expression of a viral gene by RT-PCR. Moreover, cell morphology changes by comparison of macrophage area and perimeter were determined. A second culture was obtained by cell cloning out of this culture, and a third culture was established by superinfection with the original virus, in which 92-95% of the macrophages expressed viral antigen without cell destruction and released defective particles but low levels of infectious virus. Although the three cultures maintained the characteristics of persistently infected cells, concentrations of released infectious virus, defective particles, and percentages of cells bearing viral antigen varied. RSV persistently infected murine macrophage cultures provide an in vitro model to study viral-macrophag...Continue Reading

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Citations

Apr 16, 2004·Clinical Microbiology Reviews·Andrew J EastonHelene F Rosenberg
Mar 29, 2011·Virus Research·Yuko Nakamura-LópezBeatriz Gómez
Mar 26, 2005·Canadian Journal of Physiology and Pharmacology·Gordon BolgerMichel Liuzzi
Jun 16, 2009·Cell Biology International·Yuko Nakamura-LópezBeatriz Gómez-García
May 15, 2019·Annual Review of Virology·Emmanuelle Genoyer, Carolina B López
Jun 5, 2019·Nature Microbiology·Marco Vignuzzi, Carolina B López
Feb 14, 2021·Archives of Virology·Luis Didier González-GarcíaMoisés León-Juárez

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