Characteristics of adenosine triphosphate [corrected] release from porcine and human normal bladder

The Journal of Urology
Vivek KumarRussell Chess-Williams

Abstract

Although the sensory and motor roles of the purinergic system in the bladder are well proven in animal species, there is increasing evidence that it may have an important role in humans. In addition, it may be important in the pathophysiology of bladder dysfunction. We established the level of adenosine triphosphate (ATP) release from porcine and normal human bladders. Bladder strips from patients with a urodynamically proven stable bladder undergoing surgery for stress incontinence and those undergoing cystectomy for cancer with no lower urinary tract symptoms were subjected to varying degrees of stretch (up to 50%) and electric field stimulation (10 to 40 Hz). A luciferase assay was used to quantify ATP release. Significantly increased ATP release over baseline was induced by mechanical and electrical stimulation (each p <0.05). Mean ATP release +/- SE from porcine bladders (38.2 +/- 1.9 pM/gm tissue following stretch and 19.9 +/- 6.5 pM/gm following electrical stimulation) was comparable to the release from human bladders (26.1 +/- 2.4 pM/gm tissue following stretch and 29.9 +/- 1.0 pM/gm following electrical stimulation). The main source of ATP release was the urothelium and not the muscle (p <0.05). This ATP release follow...Continue Reading

References

Mar 15, 1988·European Journal of Pharmacology·E MonferiniH Ladinsky
Jun 1, 1985·British Journal of Urology·G N Sibley
Apr 1, 1970·British Journal of Urology·E A Tanagho, E R Miller
Jul 1, 1993·The Journal of Urology·M BridgewaterA F Brading
Mar 11, 2000·British Journal of Pharmacology·M H HawthornR Chess-Williams
Nov 23, 2000·British Journal of Pharmacology·T YamanishiR Chess-Williams
Jan 15, 2002·American Journal of Physiology. Renal Physiology·G E KnightG Burnstock

❮ Previous
Next ❯

Citations

Sep 29, 2005·American Journal of Physiology. Renal Physiology·Leanne T BreenVioleta N Mutafova-Yambolieva
Apr 29, 2008·Purinergic Signalling·Michael B HovaterErik M Schwiebert
Aug 5, 2015·PloS One·Mário Angelo ClaudinoCarla Fernanda Franco-Penteado
Aug 19, 2005·American Journal of Physiology. Cell Physiology·Yan Sun, Toby C Chai
May 9, 2006·Urologia Internationalis·Massimo Lazzeri
Apr 7, 2006·American Journal of Physiology. Renal Physiology·Simon A Lewis, Jamie R Lewis
Aug 17, 2006·BJU International·Christopher R Chapple, E Ann Gormley
Feb 15, 2008·American Journal of Physiology. Renal Physiology·F Aura KullmannL A Birder
Jun 1, 2007·BJU International·Jennifer SouthgateIan Eardley
Oct 29, 2009·British Journal of Pharmacology·P SadanandaE Burcher
Feb 14, 2006·Nature Clinical Practice. Urology·Toby C Chai, Susan Keay
Jun 2, 2005·Current Opinion in Urology·Vivek KumarChristopher R Chapple
Aug 23, 2012·The Journal of Urology·Kristina D SusonJohn P Gearhart
Jun 28, 2014·BioMed Research International·Y ChengK H Moore
Nov 23, 2013·Purinergic Signalling·Geoffrey Burnstock
Mar 1, 2012·BJU International·John S YoungChristopher H Fry
Apr 12, 2006·Nature Clinical Practice. Urology·Michael R Ruggieri
Jan 28, 2014·Purinergic Signalling·Liliana RockenbachAna Maria Oliveira Battastini
Apr 19, 2011·BMC Urology·Jacqueline R WoodmanKate H Moore
May 29, 2018·Naunyn-Schmiedeberg's Archives of Pharmacology·Donna SellersMartin C Michel
May 15, 2007·Urologia Internationalis·A Erdem CandaOktay Nazli
Mar 21, 2007·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Jeroen Ronald ScheepePeter Alken

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bladder Carcinoma In Situ

Bladder Carcinoma In Situ is a superficial bladder cancer that occurs on the surface layer of the bladder. Discover the latest research on this precancerous condition in this feed.