PMID: 9161610May 1, 1997Paper

Characteristics of alcohol dehydrogenases of certain aerobic bacteria representing human colonic flora

Alcoholism, Clinical and Experimental Research
T NosovaM Salaspuro

Abstract

We have recently proposed the existence of a bacteriocolonic pathway for ethanol oxidation [i.e., ethanol is oxidized by alcohol dehydrogenases (ADHs) of intestinal bacteria resulting in high intracolonic levels of reactive and toxic acetaldehyde]. The aim of this in vitro study was to characterize further ADH activity of some aerobic bacteria, representing the normal human colonic flora. These bacteria were earlier shown to possess high cytosolic ADH activities (Escherichia coli IH 133369, Klebsiella pneumoniae IH 35385, Klebsiella oxytoca IH 35339, Pseudomonas aeruginosa IH 35342, and Hafnia alvei IH 53227). ADHs of the tested bacteria strongly preferred NAD as a cofactor. Marked ADH activities were found in all bacteria, even at low ethanol concentrations (1.5 mM) that may occur in the colon due to bacterial fermentation. The Km for ethanol varied from 29.9 mM for K. pneumoniae to 0.06 mM for Hafnia alvei. The inhibition of ADH by 4-methylpyrazole was found to be of the competitive type in 4 of 5 bacteria, and Ki varied from 18.26 +/- 3.3 mM for Escherichia coli to 0.47 +/- 0.13 mM for K. pneumoniae. At pH 7.4, ADH activity was significantly lower than at pH 9.6 in four bacterial strains. ADH of K. oxytoca, however, showed a...Continue Reading

References

Feb 1, 1977·European Journal of Biochemistry·H Jörnvall
Jul 1, 1978·Diseases of the Colon and Rectum·S BrozinskyS Wapnick
Aug 1, 1988·Alcoholism, Clinical and Experimental Research·D JacobsenK E McMartin
Jul 13, 1972·Biochimica Et Biophysica Acta·J P Tassin, J P Vandecasteele
Nov 1, 1973·Annals of Internal Medicine·F A KlipsteinW E Moore
Jul 1, 1970·The Biochemical Journal·H A Krebs, J R Perkins
Nov 1, 1982·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J H HoofnagleK Ishak
Mar 8, 1984·The New England Journal of Medicine·E S PollackS B Green
Feb 1, 1980·Archives of Biochemistry and Biophysics·K E McMartinR Blomstrand
Jan 1, 1980·Annual Review of Microbiology·J G Zeikus
Dec 1, 1994·Annals of Medicine·E Giovannucci, W C Willett
Sep 1, 1994·Gut·K JokelainenM Salaspuro
Aug 1, 1994·Alcoholism, Clinical and Experimental Research·T HaugeT Kjerstadius
Jan 1, 1994·Critical Reviews in Microbiology·M F Reid, C A Fewson
Apr 1, 1996·The Journal of Pathology·T Matysiak-BudnikM Salaspuro
May 1, 1996·Alcoholism, Clinical and Experimental Research·T Koivisto, M Salaspuro
Nov 1, 1996·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·T NosovaM Salaspuro

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Citations

Jan 16, 2014·Indian Journal of Microbiology·Himanshu KumarY S Shouche
May 27, 2004·Ground Water·Susannah K SandrinNicole T Nelson
Sep 30, 2008·Acta Odontologica Scandinavica·Jukka H Meurman, Johanna Uittamo
May 10, 2015·Medical Hypotheses·Ivanildo Coutinho de Medeiros, Josivan Gomes de Lima
May 26, 2017·International Journal of Molecular Sciences·Hye-Kyung Na, Ja Young Lee
May 13, 2006·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Farid E Ahmed
Apr 1, 2000·Addiction Biology·T Chiba, S F Phillips
Aug 3, 2000·Alcoholism, Clinical and Experimental Research·A S RosmanC S Lieber
Sep 3, 1998·Alcoholism, Clinical and Experimental Research·J P VisapääM Salaspuro
Jun 19, 2001·Alcoholism, Clinical and Experimental Research·S VäkeväinenM Salaspuro
Apr 3, 2015·Physiological Reviews·Yuri L DorokhovTatiana V Komarova

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