PMID: 6170706Dec 1, 1981Paper

Characteristics of an antigen-specific helper factor that enhances the in vitro generation of cytotoxic cells directed against a syngeneic tumor

The Journal of Immunology : Official Journal of the American Association of Immunologists
D G KilburnJ G Levy

Abstract

An antigen-specific helper factor (Hf) that enhances the in vitro generation of tumor-specific cytotoxic T cells (CL) has been isolated from the spleens of DBA/2 mice bearing the syngeneic mastocytoma, P815. This factor enhances the CL response to P815 but not to L1210 (a syngeneic T cell leukemia in DBA/2 mice), B10 minor antigens, or DBA/2 alloantigens. The factor binds specifically to P815 membrane immunoadsorbent columns and can be eluted with 2 M NaCl. P815 Hf did not resemble the lymphokine Il 2 because it did not enhance the proliferative or CL response of thymocytes nor would it support the growth of an IL 2-requiring line of T cells. This factor acted optimally if added late in the culture period in contrast to IL 2, which was more effective if added early. In limiting dilution experiments P815 Hf enhanced the level of CL activity generated but not the number of precursors triggered. IL 2 markedly amplified both of these parameters.

Related Concepts

Antigenic Specificity
Clone Cells
Immunologic Tumoricidal Activities
SD Antigens
Interleukin-1
RU 49637
Alloantigens
Leukemia L1210
Mice, Inbred C57BL
Mice, Inbred DBA

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