PMID: 11927000Apr 3, 2002Paper

Characteristics of bovine hemoglobin as a potential source of hemoglobin-vesicles for an artificial oxygen carrier

Journal of Biochemistry
Hiromi SakaiE Tsuchida

Abstract

Hemoglobin-vesicles (HbV) have been developed for use as artificial O(2) carriers in which a purified Hb solution is encapsulated within a phospholipid bilayer membrane. In this study, bovine Hb (BHb) was tested as a source of HbV instead of human Hb (HHb). We compared the preparation process and characteristics of BHbV with those of HHbV. The purification of BHb was effectively performed simply with an ultrafiltration system including a process for removing virus and scrapie reagent. The removal ratio of the phospholipid components of bovine red blood cells was over 99.99%, and the protein purity was over 99.9%. The deoxygenated and carbonylated BHb showed denaturation transition temperatures at 83 and 87 degrees C, respectively, which are higher than those of HHb (80 and 78 degrees C, respectively), and resistant to pasteurization (60 degrees C, 10 h). The purified BHb was concentrated to over 40 g/dl, and encapsulated in a phospholipid bilayer membrane to form BHbV with a diameter of about 280 nm. The O(2) affinity (P(50)) of the BHbV was regulated by coencapsulation of an appropriate amount of Cl(-) (as NaCl), which binds to BHb as an allosteric effector, in the range 16-28 Torr, comparable to human blood (P(50) = 28 Torr)....Continue Reading

Citations

Aug 3, 2012·Biomacromolecules·Kai ChenJoseph M DeSimone
Dec 17, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Shahid RameezAndre F Palmer
Apr 15, 2008·Biochimica Et Biophysica Acta·Hiromi SakaiEishun Tsuchida
Nov 17, 2009·Methods in Enzymology·Hiromi SakaiEishun Tsuchida
Oct 5, 2016·Bioscience, Biotechnology, and Biochemistry·Erina KitagawaTakashi Hayakawa
Mar 23, 2017·Artificial Organs·Kyle D WebsterKenneth W Olsen
Aug 6, 2016·Lab on a Chip·Pieter E OomenElisabeth Verpoorte
Oct 23, 2004·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Akira YoshizuKoichi Kobayashi
Mar 1, 2019·Journal of Nutritional Science and Vitaminology·Erina KitagawaTakashi Hayakawa
Mar 17, 2007·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Hiromi SakaiKoichi Kobayashi
Sep 21, 2012·Artificial Cells, Nanomedicine, and Biotechnology·Hiromi SakaiNatsuhiko Sugimura
Oct 2, 2004·The Journal of Pharmacology and Experimental Therapeutics·Keitaro SouWilliam T Phillips
Jul 26, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Hiromi SakaiEishun Tsuchida
Jul 30, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Hiromi Sakai, Cassandra Leong
Jun 18, 2005·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Dian R Arifin, Andre F Palmer
Jan 2, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Hiromi SakaiEishun Tsuchida
Jun 18, 2005·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Dian R Arifin, Andre F Palmer
May 28, 2009·The Journal of Physical Chemistry. B·Takaaki SatoEishun Tsuchida
Jun 15, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Hiromi SakaiEishun Tsuchida
Dec 15, 2007·Journal of Chromatography. a·Hans-Olof JohanssonAdalberto Pessoa
Jul 12, 2005·Biomacromolecules·Dian R Arifin, Andre F Palmer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biomaterials, Artificial Cells, and Immobilization Biotechnology : Official Journal of the International Society for Artificial Cells and Immobilization Biotechnology
A VivierD Poncelet
© 2022 Meta ULC. All rights reserved