PMID: 2500059Jul 1, 1989Paper

Characteristics of Ca2+ transport by Trypanosoma cruzi mitochondria in situ

Archives of Biochemistry and Biophysics
R Docampo, A E Vercesi

Abstract

The use of digitonin to permeabilize Trypanosoma cruzi plasma membrane has allowed the study of Ca2+ transport and oxidative phosphorylation in mitochondria in situ (R. Docampo and A. E. Vercesi (1989) J. Biol. Chem. 264, 108-111). The present results show that these mitochondria are able to build up and retain a membrane potential as indicated by a tetraphenylphosphonium-sensitive electrode. Ca2+ uptake caused membrane depolarization compatible with the existence of an electrogenically mediated Ca2+ transport mechanism in these mitochondria. Addition of Ca2+ or ethylene glycol bis (beta-aminoethyl ether) N-N'-tetraacetic acid to these preparations under steady-state conditions was followed by Ca2+ uptake or release, respectively, tending to restore the original Ca2+ "set point" at about 0.9 microM. In addition, large amounts of Ca2+ were retained by T. cruzi mitochondria even after addition of thiols and NAD(P)H oxidants such as t-butyl hydroperoxide, diamide, and the 1,2-naphthoquinone beta-lapachone. However, when ascorbate plus N,N,N',N'-tetramethyl-p-phenylenediamine in the presence of antimycin A was used as subtrate, beta-lapachone caused pyridine nucleotide oxidation, and Ca2+ accumulation by these mitochondria was cons...Continue Reading

References

Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A L LehningerE A Bababunmi
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H R LötscherC Richter
Nov 15, 1979·The Biochemical Journal·J J CannataJ J Cazzulo
Sep 27, 1976·Biochimica Et Biophysica Acta·G C Hill
Oct 6, 1975·Biochemical and Biophysical Research Communications·D SiliprandiN Siliprandi
Oct 1, 1986·British Medical Bulletin·D G Nicholls
May 1, 1987·Molecular and Biochemical Parasitology·G B HendersonA Cerami
Sep 1, 1982·Biochimica Et Biophysica Acta·D Nicholls, K Akerman
Nov 1, 1982·The Journal of Cell Biology·D L Granger, A L Lehninger
Feb 29, 1984·Biochemical and Biophysical Research Communications·A E Vercesi
Mar 1, 1984·Analytical Biochemistry·R W Moreadith, G Fiskum
Dec 30, 1981·Biochimica Et Biophysica Acta·J R WilliamsonJ B Hoek
Jul 1, 1956·Biochimica Et Biophysica Acta·M AGOSIN, E C WEINBACH

❮ Previous
Next ❯

Citations

Jul 1, 1992·Journal of Cellular Physiology·J J Blum
Aug 10, 1990·Biochimica Et Biophysica Acta·G Benaim, P J Romero
Feb 1, 1990·Molecular and Biochemical Parasitology·G BenaimJ A Urbina
Aug 1, 1990·Molecular and Biochemical Parasitology·A E VercesiR Docampo
Aug 1, 1992·Molecular and Biochemical Parasitology·A Denicola-SeoaneJ F Turrens
Feb 1, 1992·Molecular and Biochemical Parasitology·M J Walsh, J J Blum
Jun 1, 1993·Molecular and Biochemical Parasitology·R DocampoA E Vercesi
Jul 1, 1994·Molecular and Biochemical Parasitology·M A YakubuF Kierszenbaum
Dec 1, 1995·Molecular and Biochemical Parasitology·K R Santhamma, A Bhaduri
Jan 1, 1991·Parasitology Today·S A MorrisM Wittner
Dec 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M A Cataldi de Flombaum, A O Stoppani
Nov 15, 2001·Transactions of the Royal Society of Tropical Medicine and Hygiene·H W RivarolaP Paglini-Oliva
Mar 13, 1998·Molecular and Biochemical Parasitology·R BermúdezK Dawidowicz
Feb 24, 1999·Molecular and Biochemical Parasitology·L ThomsonR Radi
Feb 29, 2000·Molecular and Biochemical Parasitology·R CatistiA E Vercesi
Mar 29, 2000·The International Journal of Biochemistry & Cell Biology·C F BernardesA E Vercesi
Aug 28, 2003·Current Opinion in Microbiology·Silvia N J Moreno, Roberto Docampo
Nov 5, 2004·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Kildare MirandaRoberto Docampo
May 1, 1993·The Journal of Eukaryotic Microbiology·R DocampoA E Vercesi
Dec 3, 2013·European Journal of Pharmacology·Roberto DocampoHelmut Plattner
Jun 9, 2009·International Journal for Parasitology·Alexandre D T Costa, Marco A Krieger
Dec 4, 2014·Cell Calcium·Roberto Docampo, Guozhong Huang
Sep 15, 2014·Molecular and Biochemical Parasitology·Roberto DocampoGuozhong Huang
Jun 5, 2004·Biochimica Et Biophysica Acta·Danuza Nogueira Moysés, Hector Barrabin
Dec 30, 2017·Cell Biology International·Noelia LanderAníbal E Vercesi
Mar 7, 2020·Frontiers in Cellular and Infection Microbiology·Gustavo BenaimNathalia Martinez-Sotillo
Jun 21, 2018·Genes·Srinivasan Ramakrishnan, Roberto Docampo
Jan 21, 2021·International Journal of Molecular Sciences·Pedro H ScarpelliCelia R S Garcia
Jun 5, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Guilherme Rodrigo Rm Dos SantosRoberto Docampo
Jun 1, 1992·Experimental Parasitology·H S OzS A Morris
Sep 3, 2018·Free Radical Biology & Medicine·Anibal E VercesiEstela N B Busanello
Jul 6, 2021·Antioxidants & Redox Signaling·Roberto Docampo, Aníbal Eugénio Vercesi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.