Characteristics of Carbapenemase-Producing Enterobacteriaceae in Wastewater Revealed by Genomic Analysis

Antimicrobial Agents and Chemotherapy
Ryota GomiYasufumi Matsumura

Abstract

Wastewater is considered a major source of antibiotic-resistant bacteria released into the environment. Here, we characterized carbapenemase-producing Enterobacteriaceae (CPE) in wastewater by whole-genome analysis. Wastewater samples (n = 40) were collected from municipal wastewater treatment plants and hospital wastewater in Japan and Taiwan. Samples were screened for CPE using selective media, and the obtained isolates were sequenced using an Illumina MiSeq. The isolates (n = 45) included the following microorganisms: Klebsiella quasipneumoniae (n = 12), Escherichia coli (n = 10), Enterobacter cloacae complex (n = 10), Klebsiella pneumoniae (n = 8), Klebsiella variicola (n = 2), Raoultella ornithinolytica (n = 1), Citrobacter freundii (n = 1), and Citrobacter amalonaticus (n = 1). Among the 45 isolates, 38 harbored at least one carbapenemase-encoding gene. Of these, the blaGES (blaGES-5, blaGES-6, and blaGES-24) genes were found in 29 isolates. The genes were situated in novel class 1 integrons, but the integron structures were different between the Japanese (In1439 with blaGES-24 and In1440 with blaGES-5) and Taiwanese (In1441 with blaGES-5 and In1442 with blaGES-6) isolates. Other carbapenemase-encoding genes (blaVIM-1, bl...Continue Reading

References

Sep 6, 2003·Applied and Environmental Microbiology·Harald Hoffmann, Andreas Roggenkamp
Sep 18, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S BrisseJ Verhoef
Dec 31, 2005·Nucleic Acids Research·P SiguierM Chandler
May 1, 2007·Diagnostic Microbiology and Infectious Disease·Il Kwon BaeHasik Youn
May 8, 2007·Applied and Environmental Microbiology·Laura A BoczekJames R Johnson
Jul 17, 2007·Clinical Microbiology Reviews·Anne Marie Queenan, Karen Bush
Dec 17, 2008·Antimicrobial Agents and Chemotherapy·Ying-Tsong ChenShih-Feng Tsai
Feb 21, 2009·Bioinformatics·Alexandra MouraAntónio Correia
Oct 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Michael Richter, Ramon Rosselló-Móra
Jan 11, 2011·The Journal of Hospital Infection·T P G ChagasM D Asensi
Feb 26, 2011·Current Microbiology·Maíra Espíndola Silva de MeloAna Catarina de Souza Lopes
Mar 15, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·H StrömdahlI Odenholt
Aug 25, 2011·The Journal of Antimicrobial Chemotherapy·Masaki YamamotoSatoshi Ichiyama
Oct 11, 2011·International Journal of Medical Microbiology : IJMM·Christian-Daniel Köhler, Ulrich Dobrindt
Dec 3, 2011·Journal of Clinical Microbiology·Fu-Chen YangJiunn-Jong Wu
Apr 18, 2012·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Anton BankevichPavel A Pevzner
Apr 18, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P NordmannUNKNOWN European Network on Carbapenemases
Jul 12, 2012·The Journal of Antimicrobial Chemotherapy·Ea ZankariMette Voldby Larsen
Aug 31, 2012·Emerging Infectious Diseases·Patrice NordmannLaurent Dortet
Feb 26, 2013·BMC Bioinformatics·Jan P Meier-KolthoffMarkus Göker
Oct 4, 2013·Journal of Clinical Microbiology·Sylvain BrisseDominique Decré
Oct 5, 2013·Clinical Microbiology Reviews·Paul-Louis WoertherAntoine Andremont
Jan 1, 2014·PloS One·Cristian Del FabbroFederico M Giorgi
Apr 18, 2014·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·E RuppéS Matheron
Apr 30, 2014·Antimicrobial Agents and Chemotherapy·Alessandra CarattoliHenrik Hasman
Jun 26, 2014·MBio·Liang ChenBarry N Kreiswirth
Oct 12, 2014·Journal of Microbiological Methods·D HammoudiD Karam Sarkis
Oct 12, 2014·Trends in Microbiology·Liang ChenBarry N Kreiswirth
Feb 3, 2015·Seminars in Respiratory and Critical Care Medicine·Yohei Doi, David L Paterson
Mar 21, 2015·PloS One·Jann-Tay WangShan-Chwen Chang
Jun 24, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kathryn E HoltNicholas R Thomson

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Citations

Jul 2, 2019·Emerging Microbes & Infections·Nadia Rodríguez-MedinaUlises Garza-Ramos
Jul 25, 2019·Scientific Reports·Humberto Barrios-CamachoUlises Garza-Ramos
Apr 26, 2021·Journal of Global Antimicrobial Resistance·Zineb CherakJean-Marc Rolain
Jul 3, 2021·International Journal of Environmental Research and Public Health·Shuangfang HuYuebin Ke
Aug 30, 2021·International Journal of Hygiene and Environmental Health·Damian RolbieckiWiktor Zieliński
Sep 11, 2021·Frontiers in Cellular and Infection Microbiology·Barbara GhiglioneMilena Dropa
Nov 7, 2021·The Science of the Total Environment·Katja KehlGabriele Bierbaum

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