Characteristics of chromatin release during digestion of nuclei with micrococcal nuclease: preferential solubilization of nascent RNA at low enzyme concentration.

The International Journal of Biochemistry
D J Telford, B W Stewart

Abstract

1. Extensive digestion of nuclei with micrococcal nuclease (MNase), commonly used in the analysis of chromatin structure, results in the production of mono- and dinucleosomal chromatin fragments. 2. Digestion of nuclei from a range of cell types with low enzyme concentrations solubilized high molecular weight polynucleosomal fragments, some greater than 22 kb long. 3. Such digestion conditions also resulted in extensive solubilization of nascent RNA which contributed considerably to the nucleic acid content of the soluble fraction. 4. We conclude that the contribution of RNA to total nucleic acid content of the soluble fraction should be taken into consideration when nuclei are digested with low concentrations of MNase.

References

Jan 25, 1977·Journal of Molecular Biology·M Noll, R D Kornberg
Jul 1, 1975·Biochemistry·B Sollner-Webb, G Felsenfeld
Jan 1, 1989·The International Journal of Biochemistry·D J Telford, B W Stewart
Jul 1, 1967·The Biochemical Journal·K S Kirby, E A Cook

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Citations

Jun 15, 2007·Genome Research·Jonathan H DennisRobert E Kingston
Nov 2, 2015·Cellular and Molecular Life Sciences : CMLS·Marjo-Riitta PuumalainenHanspeter Naegeli
May 21, 2016·Frontiers in Genetics·Peter RüthemannHanspeter Naegeli

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