Characteristics of circular RNAs expression of peripheral blood mononuclear cells in humans with coronary artery disease.

Physiological Genomics
Wen-Feng JiEn-Zhi Jia

Abstract

Circular RNAs (circRNAs) function as promising biomarkers and therapeutic targets for coronary artery disease due to their high stability, covalently closed structure, and potential gene regulation. We aimed to identify the expression profile and role of circular RNAs (circRNAs) in coronary artery disease (CAD). We performed RNA sequence analysis of circRNAs in peripheral blood mononuclear cells of five patients with CAD and five controls. Bioinformatics analyses were adopted to explore biological functions of differentially expressed circRNAs. The miRanda and TargetScan tools were used to predict the microRNA (miRNA)-targeting interactions and to construct a triple network of differentially expressed gene-circRNA-miRNA-mRNA. In total, 13,160 downregulated and 12,905 upregulated circRNAs were identified in CAD. A gene ontology annotation analysis showed that genes in the network were involved in organelle organization, cell cycle, mitotic cycle, and cellular metabolic process. Parental genes of the 10 dysregulated circRNAs were involved in metabolism and protein modification, and these circRNAs might regulate gene expression associated with CAD via miRNA sponges. As potential competing endogenous RNAs (ceRNAs), dysregulated cir...Continue Reading

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Methods Mentioned

BETA
coronary artery bypass
density gradient centrifugation
Illumina sequencing
RNA-seq
PCR

Software Mentioned

edgeR
STAR
cutadapt
R heatmap . 2
DCC
Cytoscape
miRanda
TargetScan

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