PMID: 7032591Dec 7, 1981Paper

Characteristics of dipeptide transport in normal and papain-treated brush border membrane vesicles from mouse intestine. I. Uptake of glycyl-L-phenylalanine

Biochimica Et Biophysica Acta
A BertelootK Ramaswamy

Abstract

Papain treatment of isolated brush border membrane vesicles was carried out to study peptide transport in the absence of hydrolytic events associated with the brush border membrane. Such a treatment allowed a 70% decrease in the activity of membrane-associated oligopeptidases and the study of peptide transport in the complete absence of free amino acids up to 1 min of incubation. A comparison between the time course curves of glycyl-L-phenylalanine uptake by normal and papain-treated vesicles showed that the overshoots seen in the presence of Na+ and K+ gradients (extravesicular greater than intravesicular) when using normal vesicles were no longer evident after papain treatment. This result, together with the demonstration of uptake into an osmotically reactive intravesicular space and the analysis of uptake of free phenylalanine, allowed the conclusion that peptide transport was the result of two complementary mechanisms, uptake of free amino acids following hydrolysis by the membrane-bound oligopeptidases, and intact peptide transport down a concentration gradient by a non-Na+ (and non-K+)-dependent process. These results also showed the non-involvement of gamma-glutamyltransferase and the gamma-glutamyl cycle in peptide abs...Continue Reading

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Citations

Jan 1, 1988·Comparative Biochemistry and Physiology. A, Comparative Physiology·R Kushak, N Basova
Apr 17, 1999·International Journal of Pharmaceutics·C L StevensonR W Hendren
Feb 14, 1985·Biochimica Et Biophysica Acta·C I Cheeseman, D Devlin
Jul 7, 1988·Biochimica Et Biophysica Acta·M T VincenziniT Iantomasi
Oct 2, 1987·Biochimica Et Biophysica Acta·J M HarigK Ramaswamy
Mar 28, 1985·Biochimica Et Biophysica Acta·N TakuwaT Hoshi
Dec 1, 1988·Journal of Pharmaceutical Sciences·P M Bummer, G Zografi
Sep 28, 1984·Biochemical and Biophysical Research Communications·M LinderP Sudaka
May 21, 2003·American Journal of Veterinary Research·Randal K Buddington, Christiane Malo
Apr 18, 2008·The Journal of Pharmacy and Pharmacology·Matthias BrandschEva Bosse-Doenecke
Jan 1, 1989·Nutrition Research Reviews·G K Grimble, D B Silk
Nov 12, 2003·The Journal of Pharmacology and Experimental Therapeutics·Xueying YanQiang Zhang
Jun 21, 1982·Life Sciences·V Ganapathy, F H Leibach
Dec 19, 1984·Biochimica Et Biophysica Acta·V M RajendranK Ramaswamy
Nov 17, 1989·Biochimica Et Biophysica Acta·D WilsonK Ramaswamy

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