Abstract
Cell death induced by etoposide in the human lymphoma cell line U-937 GTB was characterized. Activity of caspases -3, -8 and -9 was measured by spectrophotometric detection of specific cleavage products, DNA fragmentation by TdT-mediated dUTP nick end-labelling (TUNEL), and apoptotic morphology by conventional staining and microscopy, as well as by a novel method-the microculture kinetics (MiCK) assay. Synthesis of protein and DNA during exposure was monitored by incorporation of radioactive leucine and thymidine, respectively. The effects of caspase inhibitors on total viability, as well as early and late morphological changes were studied. Etoposide rapidly induced apoptosis, dependent on caspase-3 and -8, but inhibition of these caspases did not prevent major cell death, but promoted a switch in late morphology. The novel MiCK assay added valuable information on early morphological events during cell death. Hence, this study provides support for caspase-8-mediated apoptosis in U-937 GTB when exposed to etoposide. General caspase inhibition switches cell death to one with a different morphology.
References
May 15, 1976·International Journal of Cancer. Journal International Du Cancer·C Sundström, K Nilsson
Sep 1, 1992·Cancer Metastasis Reviews·J A Hickman
Jan 21, 1992·International Journal of Cancer. Journal International Du Cancer·R LarssonP Nygren
Aug 1, 1972·British Journal of Cancer·J F KerrA R Currie
Apr 15, 1994·Cancer·J F KerrB V Harmon
Dec 11, 1995·FEBS Letters·L GhibelliG Gualandi
Dec 26, 1995·Biochemical and Biophysical Research Communications·T MashimaT Tsuruo
Mar 15, 1996·Experimental Cell Research·L DiniL Ghibelli
Jan 1, 1996·Cancer Treatment and Research·S G Lutzker, A J Levine
Dec 15, 1996·Analytical Biochemistry·D W HarrisD A Jones
Jun 1, 1997·Analytical Biochemistry·R GravesN Cook
Feb 28, 1998·The EMBO Journal·E Bossy-WetzelD R Green
Jun 4, 1998·Biochemical and Biophysical Research Communications·N Fujita, T Tsuruo
Sep 28, 1998·Biochimica Et Biophysica Acta·S H Kaufmann
Jan 16, 1999·Molecular and Cellular Biology·Z HanJ H Wyche
Jan 20, 1999·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·K R Hande
Jan 23, 1999·Oncogene·G NuñezN Inohara
Feb 13, 1999·The Journal of Biological Chemistry·X M SunG M Cohen
Apr 14, 1999·Cell Death and Differentiation·A G Porter, R U Jänicke
Oct 9, 1999·The American Journal of Pathology·V D KravtsovM J Koury
Nov 2, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C GarridoE Solary
Nov 5, 1999·Structure·G S Salvesen
Nov 11, 1999·Leukemia·C FriesenK M Debatin
Feb 25, 2000·The International Journal of Biochemistry & Cell Biology·K Kuida
Mar 31, 2000·Experimental Cell Research·S H Kaufmann, W C Earnshaw
Apr 11, 2000·Anti-cancer Drugs·P ShrivastavaP Ranjan
Jun 29, 2000·Annual Review of Biochemistry·W C EarnshawS H Kaufmann
Jul 19, 2000·FEBS Letters·V E KaganK Kawai
Citations
Mar 23, 2004·Gene·Mingxu XuDalong Ma
Jun 1, 2005·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Carlo Dell'ErbaMaurizio Viale
Oct 3, 2002·British Journal of Pharmacology·P MartinssonR Larsson
May 18, 2005·Oncogene·Bernard L MirkinAbdelhadi Rebbaa
Aug 21, 2002·Anti-cancer Drugs·B-M FrostE Lindhagen
Nov 23, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Valentina YurinskayaAlexey Vereninov
Aug 27, 2013·PloS One·Karen CawleyAfshin Samali
Mar 30, 2012·PLoS Pathogens·Amit SharmaKathleen Boris-Lawrie
Nov 13, 2007·Biochemical Pharmacology·Maria E RiveiroCarina Shayo
Sep 4, 2004·Journal of Biochemical and Biophysical Methods·John FlensburgDaniel Ivansson
Aug 27, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Valentina E YurinskayaAlexey A Vereninov
Jul 10, 2016·Methods : a Companion to Methods in Enzymology·Ivan A Vorobjev, Natasha S Barteneva
Dec 7, 2007·Bioorganic & Medicinal Chemistry·Maria E RiveiroCarlos Davio
Nov 18, 2009·ChemMedChem·Przemysław ReszkaPatrick J Bednarski
May 14, 2003·Oncogene·Abdelhadi RebbaaBernard L Mirkin
Oct 4, 2005·The British Journal of Nutrition·Lisa RyanNora M O'Brien
Oct 20, 2004·Cancer Research·Marina ZanonAndrea Anichini
Mar 5, 2004·Cancer Research·Xin ZhengAbdelhadi Rebbaa