PMID: 11604558Oct 18, 2001Paper

Characteristics of etoposide-induced apoptotic cell death in the U-937 human lymphoma cell line

Anti-cancer Drugs
P MartinssonR Larsson

Abstract

Cell death induced by etoposide in the human lymphoma cell line U-937 GTB was characterized. Activity of caspases -3, -8 and -9 was measured by spectrophotometric detection of specific cleavage products, DNA fragmentation by TdT-mediated dUTP nick end-labelling (TUNEL), and apoptotic morphology by conventional staining and microscopy, as well as by a novel method-the microculture kinetics (MiCK) assay. Synthesis of protein and DNA during exposure was monitored by incorporation of radioactive leucine and thymidine, respectively. The effects of caspase inhibitors on total viability, as well as early and late morphological changes were studied. Etoposide rapidly induced apoptosis, dependent on caspase-3 and -8, but inhibition of these caspases did not prevent major cell death, but promoted a switch in late morphology. The novel MiCK assay added valuable information on early morphological events during cell death. Hence, this study provides support for caspase-8-mediated apoptosis in U-937 GTB when exposed to etoposide. General caspase inhibition switches cell death to one with a different morphology.

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Citations

Nov 23, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Valentina YurinskayaAlexey Vereninov
Sep 4, 2004·Journal of Biochemical and Biophysical Methods·John FlensburgDaniel Ivansson
Aug 27, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Valentina E YurinskayaAlexey A Vereninov
Jul 10, 2016·Methods : a Companion to Methods in Enzymology·Ivan A Vorobjev, Natasha S Barteneva
Dec 7, 2007·Bioorganic & Medicinal Chemistry·Maria E RiveiroCarlos Davio
May 14, 2003·Oncogene·Abdelhadi RebbaaBernard L Mirkin
Mar 5, 2004·Cancer Research·Xin ZhengAbdelhadi Rebbaa

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