Characteristics of GABA-activated chloride channels in mammalian dorsal root ganglion neurones.

The Journal of Physiology
B Robertson

Abstract

1. The properties of gamma-aminobutyric acid (GABA)-activated chloride channels in dorsal root ganglion (DRG) neurones obtained from rats and cats were examined using the single-electrode voltage clamp in conjunction with suction-electrode techniques. 2. GABA-evoked currents showed voltage-sensitive kinetics. Time constants (tau D) were measured from voltage-jump relaxations and tau D became briefer with membrane hyperpolarization. tau D was 33 ms at -120 mV with 60 microM-GABA and changed e-fold for 188 mV. tau D decreased as GABA concentration was increased - the extrapolated tau D at 'zero' GABA concentration was approximately equal to 50 ms at -120 mV. 3. The steady-state current in GABA was curvilinear, rectifying at negative potentials. The instantaneous current was linear with symmetrical chloride concentrations (140 mM) on both sides of the cell membrane. 4. Muscimol was a more effective agonist than GABA, while piperidine-4-sulphonic acid and ethylenediamine monocarbamate were only weakly effective agonists. Taurine and glycine had no detectable agonist activity. 5. Ion substitution experiments revealed the permeability sequence I- greater than Br- greater than Cl- greater than F- greater than propionate (1.88 greater ...Continue Reading

Citations

Sep 1, 1994·Journal of Neurocytology·V Shinder, M Devor
Apr 27, 2007·Journal of Neurophysiology·Kareem A ZaghloulJonathan B Demb
Nov 19, 2015·Proceedings of the National Academy of Sciences of the United States of America·Oleg A SineshchekovJohn L Spudich
Apr 5, 2017·The Journal of Clinical Investigation·Xiaona DuNikita Gamper
Apr 30, 2020·Science Signaling·Shihab ShahNikita Gamper
Apr 24, 2020·Frontiers in Neuroscience·Bettina U WilkeMichaela Kress
Jun 1, 1997·Progress in Neurobiology·E Kumamoto
Mar 1, 1994·Progress in Neurobiology·K Kaila

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