PMID: 4061449Nov 1, 1985Paper

Characteristics of hexokinase, pyruvate kinase, and glucose-6-phosphate dehydrogenase during adult and neonatal reticulocyte maturation

American Journal of Hematology
G JansenG E Staal

Abstract

Erythrocytes from adults and newborn infants (at term and premature) were separated by Percoll density gradient centrifugation into four fractions of increasing density. Glycolytic enzymes, especially the age-dependent ones, hexokinase (EC 2.7.1.1, HK), pyruvate kinase (EC 2.7.1.40, PK), and glucose-6-phosphate dehydrogenase (EC 1.1.1.49, G6PD) were studied during reticulocyte maturation and further red cell senescence. Analysis of the fraction with lowest density showed an almost linear and steep decline of HK, PK, and G6PD activity with a decreasing number of reticulocytes. In the next three fractions of increasing density, the activity decline was far less. These data are therefore illustrative for a biphasic activity decay pattern of HK, PK, and G6PD during both adult and neonatal red cell aging. The strong decline in HK activity could not be ascribed to the disappearance of a particulate (mitochondrial) bound fraction of the enzyme during reticulocyte maturation. All hexokinase activity in human reticulocytes was found to be cytosolic in contrast with rabbit reticulocytes in which 70% of HK activity was particulate.

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Citations

Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M Inaba, Y Maede
May 22, 2002·Mechanisms of Ageing and Development·W HildebrandtW Dröge
Jun 1, 2002·Ageing Research Reviews·Wulf Dröge
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Jan 31, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Suttiphan KitcharoenChanudda Kleesuk
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Nov 13, 1991·Biochimica Et Biophysica Acta·A R Beaudoin, G Grondin
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