PMID: 4061449Nov 1, 1985Paper

Characteristics of hexokinase, pyruvate kinase, and glucose-6-phosphate dehydrogenase during adult and neonatal reticulocyte maturation

American Journal of Hematology
G JansenG E Staal


Erythrocytes from adults and newborn infants (at term and premature) were separated by Percoll density gradient centrifugation into four fractions of increasing density. Glycolytic enzymes, especially the age-dependent ones, hexokinase (EC, HK), pyruvate kinase (EC, PK), and glucose-6-phosphate dehydrogenase (EC, G6PD) were studied during reticulocyte maturation and further red cell senescence. Analysis of the fraction with lowest density showed an almost linear and steep decline of HK, PK, and G6PD activity with a decreasing number of reticulocytes. In the next three fractions of increasing density, the activity decline was far less. These data are therefore illustrative for a biphasic activity decay pattern of HK, PK, and G6PD during both adult and neonatal red cell aging. The strong decline in HK activity could not be ascribed to the disappearance of a particulate (mitochondrial) bound fraction of the enzyme during reticulocyte maturation. All hexokinase activity in human reticulocytes was found to be cytosolic in contrast with rabbit reticulocytes in which 70% of HK activity was particulate.


Jul 1, 1975·The Journal of Pediatrics·M Komazawa, F A Oski
Nov 1, 1979·Pediatric Research·M GahrW Schröter
Jan 1, 1977·American Journal of Hematology·S H ChenG Stamatoyannopoulos
Oct 1, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·G RijksenG E Staal
Oct 15, 1979·Clinica Chimica Acta; International Journal of Clinical Chemistry·C M RennieA Maddy
Dec 15, 1975·Clinica Chimica Acta; International Journal of Clinical Chemistry·P A RogersH Harris
Jan 19, 1974·Clinica Chimica Acta; International Journal of Clinical Chemistry·B M TurnerH Harris
Jun 1, 1983·American Journal of Hematology·S Miwa
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·E Beutler
Mar 1, 1982·British Journal of Haematology·A N LestasJ M White
Jul 1, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·G SalvoA M Salvati
Jan 1, 1981·American Journal of Hematology·D E PagliaW N Valentine
Feb 1, 1980·British Journal of Haematology·J W AkkermanG C Christiaens
Sep 1, 1980·Biochimica Et Biophysica Acta·R J KraaijenhagenG E Staal

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