PMID: 6166701Mar 1, 1981Paper

Characteristics of human large granular lymphocytes and relationship to natural killer and K cells

The Journal of Experimental Medicine
T TimonenR B Herberman

Abstract

Recent evidence, has demonstrated an association between a subpopulation of peripheral blood mononuclear cells, morphologically identified as large granular lymphocytes (LGL), and natural killer (NK) activity. We have now evaluated more directly the role of LGL in both NK activity and antibody- dependent cellular cytotoxicity (ADCC), by using highly enriched populations of LGL, obtained by centrifugation of peripheral blood mononuclear cells on Percoll discontinuous density gradients. Both spontaneous and interferon- augmented NK and ADCC activities were exclusively associated with the LGL- enriched, low density fractions. The majority of LGL formed conjugates with NK-susceptible and antibody-coated target cells. Approximately 20 percent of small conventional lymphocytes also formed conjugates with the target cells for NK, but this was not associated with cytotoxic activity. Virtually all LGL were found to have receptors for the Fc portion of IgG (FcgammaR). The frequency of LGL among blood leukocytes was 2-6 percent. LGL could be enriched to an average purity of 95 percent by combining discontinuous density gradient centrifugation with subsequent adsorptions of the low density fractions on monolayers of immobilized immune comp...Continue Reading

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Citations

Jun 11, 1999·International Journal of Cancer. Journal International Du Cancer·M OhsawaK Aozasa
Jan 1, 1990·Journal of Clinical Laboratory Analysis·T L WhitesideR B Herberman
Jan 1, 1987·Cancer Metastasis Reviews·J R Ortaldo
Jan 1, 1983·Cancer Metastasis Reviews·R K Oldham
Jan 1, 1983·Cancer Metastasis Reviews·M Introna, A Mantovani
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Jan 1, 1989·Anatomy and Embryology·P Groscurth
Mar 1, 1982·Immunogenetics·X ChardonnensM Jeannet
Feb 1, 1991·Mycopathologia·R A Lindemann, C K Franker
Apr 1, 1986·Biulleten' eksperimental'noĭ biologii i meditsiny·V A Matveeva, T E Kliuchareva
Sep 1, 1983·Biulleten' eksperimental'noĭ biologii i meditsiny·T E Kliuchareva, V A Matveeva
Jun 1, 1987·Biulleten' eksperimental'noĭ biologii i meditsiny·V V MalaĭtsevV S Zueva
Jan 1, 1985·Journal of Clinical Immunology·R KiblerJ F Jones
Jan 1, 1986·Journal of Clinical Immunology·B A BlazarJ A Mannick
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Jan 1, 1986·Immunologic Research·L A Holmberg, K A Ault
Mar 5, 2013·Archivum Immunologiae Et Therapiae Experimentalis·Paweł PiątkiewiczPiotr Ciostek

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