Characteristics of macrophage-derived foam cells isolated from atherosclerotic lesions of rabbits

Atherosclerosis
M NaitoA Iguchi

Abstract

It is generally accepted that the foam cells in atherosclerotic lesions are derived mainly from monocytes/macrophages. We investigated whether the macrophage-derived foam cells, isolated from the atherosclerotic lesions of cholesterol-fed rabbits, would exhibit properties similar to those of blood monocytes in vitro and whether the cholesterol concentration of the macrophage-derived foam cells would decrease in the presence of an appropriate cholesterol acceptor in culture. We found that most (> 98%) of the foam cells isolated from atherosclerotic lesions were positive for anti-monocyte-macrophage antibody and nonspecific esterase. While almost all (> 98%) of the foam cells exhibited NaF-resistant, nonspecific esterase activity, the blood monocytes exhibited no such activity. Macrophage-derived foam cells contained larger amounts of cholesterol, most of it esterified, than the blood monocytes. Although blood monocytes exhibited a substantial amount of lysozyme, the freshly isolated, macrophage-derived foam cells showed no detectable lysozyme activity. The production of superoxide by macrophage-derived foam cells stimulated by PMA or opsonized zymosan was lower than that of stimulated monocytes. The cholesterol concentration of ...Continue Reading

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Citations

Jan 9, 2007·Redox Report : Communications in Free Radical Research·David M van ReykWendy Jessup
Oct 18, 2015·Biochemical and Biophysical Research Communications·Sun-Mi KimKoanhoi Kim
Feb 20, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Vahitha B Abdul-SalamRobert J Edwards
Dec 5, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Eun A KoErnesto L Schiffrin

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