PMID: 6972975Jan 1, 1981Paper

Characteristics of nylon fiber adherence-separated chicken splenocytes

Journal of Immunological Methods
S J Lamont, P J Val Alten

Abstract

Separation of chicken splenocytes by nylon fiber adherence yielded two distinct subpopulations. The non-adherent cells responded well to T cell mitogens, while the recoverable adherent cells did not. The non-adherent subpopulation showed a marked decrease in the number of surface immunoglobulin-bearing cells as compared to the adherent population. Cell diameter distribution was skewed toward larger sizes in the adherent subpopulation, and smaller sizes in the non-adherent subpopulation. There was no significant difference between the groups, however, in the number of cells which bore Fc receptors, stained for non-specific esterase, or actively phagocytosed. Therefore, the non-adherent subpopulation appeared to be enriched for T cells, the adherent subpopulation for B cells, and neither group for macrophages.

References

Jan 1, 1979·Journal of Immunological Methods·A CorriganH Smyth
Jul 1, 1978·Journal of Clinical Pathology·J T WozniakJ Stuart
Jan 1, 1977·Journal of Immunological Methods·T SabetP Van Alten
Oct 1, 1973·European Journal of Immunology·M H JuliusL A Herzenberg
Jan 1, 1973·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C Y LiL T Yam

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Citations

Jul 1, 1984·Avian Pathology : Journal of the W.V.P.A·J M Sharma, I Tizard
May 1, 1982·Clinical Immunology and Immunopathology·S A Darby, P J Van Alten
Nov 10, 2020·Analytica Chimica Acta·Kenichi Nagase, Hideko Kanazawa

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