Nov 4, 2009

Characteristics of PBMC obtained from leukapheresis products and tumor biopsies of patients with non-small cell lung cancer

Oncology Reports
John R YannelliEdward A Hirschowitz

Abstract

The current study characterized peripheral blood mononuclear cells (PBMC) obtained from leukapheresis products of patients with non-small cell lung cancer (NSCLC) for cytokine release, the ability to incorporate tritiated thymidine following stimulation using PHA as well as the levels of both CD4 and CD8 regulatory T cells (Tregs) as defined by FoxP3 expression. Results were compared to normal donor PBMC obtained from buffy coat products. Heterogeneous levels of Th1 (gamma interferon and IL-2), Th2 (IL-10 and IL-13), pro-inflammatory (TNF-alpha and IL-6) and the hematopoietic inducing cytokine GMCSF were detected from both populations of PBMC as measured using ELISA. Overall, we observed that combined levels of Th1 and Th2 cytokines were higher in lung cancer patients compared to that seen in normal donor PBMC. The increased cytokine production was coupled with an observed decrease in the ability of lung cancer patient PBMC to incorporate tritiated thymidine. Furthermore, cytokine containing supernatants obtained from patients inhibited the incorporation of tritiated thymidine from PBMC obtained from normal donors. Thus, the combined cytokines which included high levels of IL-10, appeared to exhibit suppressive functional activ...Continue Reading

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Mentioned in this Paper

Leukocyte Buffy Coat
Immune Response
Immune System
Tumor Necrosis Factor-alpha
Thymidine
Granulocyte Macrophage Colony Stimulating Factor Measurement
CSF2 wt Allele
Peripheral Blood Mononuclear Cells (Lab Test)
Granulocyte-Macrophage Colony-Stimulating Factor
Recombinant Interleukin-10

About this Paper

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