PMID: 15344206Sep 3, 2004Paper

Characteristics of potassium and calcium currents of hepatic stellate cells (ito) in rat

Yonsei Medical Journal
Keon Il LeeBoe Gwun Chun

Abstract

Hepatic stellate cells (HSCs) are known to play a role in the pathogenesis of the increased intrahepatic vascular resistance found in chronic liver diseases. The aim of this study was to evaluate the K+ and Ca2+ currents in cultured HSCs from rat liver, through the patch-clamp technique. Most cells were positive for desmin immunostain after isolation and in alpha-smooth muscle actin immunostain after 10 - 14 days of culturing. Outward and inward rectifying K+ currents were confirmed. Two different types of K+ currents were distinguished: one with the inward rectifying current and the other without. The outward K+ currents consisted of at least four components: tetraethylammonium (TEA)-sensitive current, 4-aminopyridine (4-AP)-sensitive current, pimozide-sensitive current and three blocker-resistant current. The peaks of the outward K+ currents evoked by a depolarizing pulse were decreased to 32.0 +/- 3.0, 62.8 +/- 3.7 and 32.8 +/- 3.5% by 5 mM TEA, 2 mM 4-AP and 15 micro M pimozide, respectively. Moreover, the combined application of three blockers caused 86.6 +/- 4.8% suppression. The inward currents evoked hyperpolarizing pulses were inwardly rectifying and almost blocked by Ba2+. Elevation of external K+ increased the inward...Continue Reading

Citations

Mar 1, 2012·Liver International : Official Journal of the International Association for the Study of the Liver·Moon Young KimSei Jin Chang
Sep 13, 2014·Liver International : Official Journal of the International Association for the Study of the Liver·Christian FreiseRajan Somasundaram
Jun 27, 2008·Yonsei Medical Journal·Dong Hyeon LeeKyu-Sang Park
Aug 28, 2007·Yonsei Medical Journal·Dong Hyeon LeeBok Ghee Han

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