Characteristics of rat bone marrow cells differentiated into a liver cell lineage and dynamics of the transplanted cells in the injured liver

Journal of Gastroenterology
Kazuo OkumotoSumio Kawata

Abstract

Bone marrow cells (BMCs) have been shown to differentiate into a liver cell lineage, but little is known about their dynamics following transplantation. BMCs were cultured to investigate the expression of liver-specific genes in vitro and transplanted into in vivo liver-injury models to elucidate their dynamics in the liver. The mRNA expression of various liver-specific genes in BMCs cocultured with hepatocytes was analyzed using reverse transcription-polymerase chain reaction. BMCs from transgenic rats expressing green fiuorescent protein were transplanted into the spleen of rat liver-injury models induced with 2-acetylaminofiuorene (2-AAF) or carbon tetrachloride (CCl4). BMCs were also transplanted directly into livers treated with CCl4 to determine which route is better for transplantation. BMCs differentiated into a liver cell lineage in vitro and expressed mRNAs consistent with mature hepatocytes, including albumin. The transplanted BMCs were found in the liver in the CCl4-induced injury model, but not in the 2-AAF-induced model. The hepatocyte growth factor and fibroblast growth factor mRNA levels in the liver were significantly higher in the CCl4-induced model than in the 2-AAF-induced model. Migration of BMCs to the liv...Continue Reading

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Citations

Apr 18, 2008·World Journal of Gastroenterology : WJG·Hu LinLi Jiang
Aug 16, 2012·Hepatobiliary & Pancreatic Diseases International : HBPD INT·Xu-Bo Wu, Ran Tao
Sep 23, 2014·World Journal of Methodology·Takafumi SaitoYoshiyuki Ueno
Feb 28, 2006·Journal of Gastroenterology·Isao Sakaida

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