Nov 1, 1976

Characteristics of RNA isolated from the nuclear RNA-synthesizing complex of influenza virus

Voprosy virusologii
T A Asadullaev, A G Bukrinskaia


When Ehrlich ascitic carcinoma cells infected with classical fowl plague virus and treated with actinomycin D were pulse labeled for 10 min with 3H-uridine, it was mainly incorporated into nucleoplasm structures sedimenting in sucrose gradients at 120S. At 2-hr exposure of the infected cells to 3H-uridine radioactivity was found in nucleoplasm in the area of 65S and in the cytoplasm in 30-40S zone. The analysis of RNA isolated from these structures gave the following results. The RNA isolated from 120S structures sedimented in two zones of sucrose gradient: 11S and 16-23S. The 11S RNA was resistant to RNA-ase, while 16-23S RNA was sensitive to RNA-ase. A similar (16-23S) RNA was isolated from virus-specific structures 65S and 30-40S.

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Mentioned in this Paper

Centrifugation, Density Gradient
Transcription, Genetic
Virus Replication
Cell Nucleus
Influenza A Virus, Avian
Alkaline Ribonuclease
Orthomyxovirus Type A, Porcine
Carcinoma, Ehrlich Tumor

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